AB231729

Anti-ATP4A 抗体

Name: Anti-ATP4A 抗体 (ab231729) | Abcam
Brand: Abcam Limited
SKU: AB231729
Price: 74000 JPY
Availability: InStock

Anti-ATP4A antibody

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(3 Publications)

Rabbit Polyclonal ATP4A antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 3 publications.

別名を表示する

Potassium-transporting ATPase alpha chain 1, Gastric H(+)/K(+) ATPase subunit alpha, Proton pump, Atp4a

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP4A antibody (AB231729)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP4A antibody (AB231729)

Formalin-fixed, paraffin-embedded mouse stomach tissue stained for ATP4A using ab231729 at 20 μg/mL in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-ATP4A antibody (AB231729)

All lanes:

Western blot - Anti-ATP4A antibody (ab231729) at 2 µg/mL

All lanes:

Recombinant mouse ATP4a protein

Predicted band size: 114 kDa

false

Supplier Data

Western blot - Anti-ATP4A antibody (AB231729)

All lanes:

Western blot - Anti-ATP4A antibody (ab231729) at 3 µg/mL

Lane 1:

Mouse heart tissue

Lane 2:

Mouse brain tissue

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 114 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse

アプリケーション

IHC-P, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

精製に関する特記事項

Followed by Protein A affinity chromatography.

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ATP4A also known as the gastric H+/K+ ATPase alpha subunit is an important enzyme in the gastric lining. It is a P-type ATPase with a molecular mass of approximately 100 kDa. ATP4A primarily expresses in the parietal cells of the stomach where it contributes to the secretion of hydrochloric acid. This alpha subunit forms a catalytic core that cooperates with the beta subunit to actively transport ions across the stomach cell membrane.

Biological function summary

ATP4A facilitates the acidification process necessary for digestion. It operates as part of the gastric proton pump complex. This complex exchanges K+ ions from the gastric lumen with H+ ions from within the parietal cell cytoplasm driving the generation of stomach acid. The enzyme's action is central to the maintenance of the acidic pH in the stomach which is important for proper protein digestion and absorption of various nutrients.

Pathways

ATP4A plays a major role in the gastric acid secretion pathway. This pathway includes interactions with the carbonic anhydrase enzyme which provides the necessary bicarbonate ions to initiate the acidification process. ATP4A also forms interactions with the Na+/K+ ATPase as a part of ion balance and energy supply ensuring that the ionic gradients are appropriately established for gastric functions.

ATP4A's function is directly related to conditions like peptic ulcer disease and gastric cancer. Hyperactivity of ATP4A can lead to excessive acid secretion contributing to peptic ulcers. Additionally its chronic overexpression may have links to gastric cancer through persistent mucosal damage. Research also explores its association with other proteins like the epidermal growth factor receptor (EGFR) which can influence gastric tissue proliferation and healing processes.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

The catalytic subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells (PubMed : 7762614). Uses ATP as an energy source to pump H(+) ions to the gastric lumen while transporting K(+) ion from the lumen into the cell (PubMed : 7762614). Remarkably generates a million-fold proton gradient across the gastric parietal cell membrane, acidifying the gastric juice down to pH 1 (PubMed : 10764766). Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). The release of the H(+) ion in the stomach lumen is followed by binding of K(+) ion converting the pump conformation back to the E1 state (By similarity) (PubMed : 7762614).

See full target information Atp4a

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular gastroenterology and hepatology 19:101448 PubMed39943905

2025

The Protective Role of DDIT4 in Helicobacter pylori-induced Gastric Metaplasia Through Metabolic Regulation of Ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Wang,Xinbo Xu,Yaobin Ouyang,Xiao Fei,Cong He,Xianhe Yang,Yuping Ren,Yanan Zhou,Sihai Chen,Yi Hu,Jianping Liu,Zhongming Ge,William Ka Kei Wu,Nonghua Lu,Chuan Xie,Xidong Wu,Yin Zhu,Nianshuang Li

Annals of translational medicine 10:218 PubMed35280359

2022

Panax notoginseng attenuates hypoxia-induced glycolysis in colonic mucosal epithelial cells in DSS-induced colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei He,Huafeng Pan,Ping Tao,Jiang Lin,Beiping Zhang,Shiying Wang

Computational and mathematical methods in medicine 2021:9932088 PubMed34367321

2021

The High Expression of PTPRH Is Associated with Poor Prognosis of Human Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Aifeng Chen,Shibiao Ding,Xiaoqiang Shen,Xuai Lin

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP4A 抗体

Anti-ATP4A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP4A antibody (AB231729)

Western blot - Anti-ATP4A antibody (AB231729)

Western blot - Anti-ATP4A antibody (AB231729)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

Abcam product promise