AB137055

Anti-ATP1B3 抗体 [EPR8981]

Anti-ATP1B3 antibody [EPR8981]

RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(10 Publications)

Rabbit Recombinant Monoclonal ATP1B3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 10 publications.

別名を表示する

CD298, Sodium/potassium-transporting ATPase subunit beta-3, Sodium/potassium-dependent ATPase subunit beta-3, ATPB-3, ATP1B3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (AB137055)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (AB137055)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling ATP1B3 with ab137055 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (AB137055)

Immunohistochemical analysis of paraffin-embedded Human cervical squamous carcinoma tissue labelling ATP1B3 with ab137055 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ATP1B3 antibody [EPR8981] (AB137055)

All lanes:

Western blot - Anti-ATP1B3 antibody [EPR8981] (ab137055) at 1/1000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 32 kDa

Observed band size: 40 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR8981

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ATP1B3 also known as ATPase Na+/K+ transporting subunit beta-3 is a protein involved in ion transport. It has a molecular mass of around 31 kDa. ATP1B3 is part of the Na+/K+ ATPase complex which maintains electrochemical gradients across cell membranes by pumping sodium out of and potassium into the cell using ATP as energy source. It is widely expressed in different tissues with notable expression in the kidneys brain and heart. This protein plays a functional role by serving as an accessory subunit that stabilizes and regulates the enzyme complex.

Biological function summary

ATP1B3 supports the proper functioning of the Na+/K+ ATPase by influencing the conformation and activity of the enzyme complex. The protein ensures that the ion gradients needed for basic cellular activities such as electrical excitability and nutrient uptake are preserved. It along with alpha subunits forms heterodimeric complexes required for ion exchange. Its presence ensures that the complex works effectively supporting various physiological functions across multiple organ systems.

Pathways

It aligns with the ion transport and signal transduction pathways related to maintaining cellular homeostasis. ATP1B3 interacts with other proteins like the alpha-1 subunit of the Na+/K+ ATPase important for regulating membrane potentials. The protein plays a role in the Wnt signaling pathway influencing processes such as cell proliferation and migration. Understanding ATP1B3 in these pathways helps unveil its broad functional implications.

ATP1B3 associates with both cardiovascular diseases and neurological disturbances. Mutations or altered expression of this protein disrupt ion exchange which can lead to heart failure or epilepsy. In these conditions blurred regulation of ATP1B3 affects the Na+/K+ ATPase complex composed of alpha and gamma subunits. This can result in imbalanced ion homeostasis emphasizing the importance of ATP1B3 in maintaining normal physiological conditions.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known.

See full target information Sodium/potassium-transporting ATPase subunit beta-3

style

left-column

文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:121 PubMed38167556

2024

Unraveling the surface marker signature of cell-derived vesicles via proteome analysis and nanoparticle flow cytometry.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Chong Lau,Ilaria Passalacqua,Jik-Han Jung,Yerim Kwon,Davide Zocco,Sung-Soo Park,Seung Wook Oh

Nature communications 13:2167 PubMed35443762

2022

Single cell transcriptomic analysis reveals cellular diversity of murine esophageal epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Faujul Kabir,Adam L Karami,Ricardo Cruz-Acuña,Alena Klochkova,Reshu Saxena,Anbin Mu,Mary Grace Murray,Jasmine Cruz,Annie D Fuller,Margarette H Clevenger,Kumaraswamy Naidu Chitrala,Yinfei Tan,Kelsey Keith,Jozef Madzo,Hugh Huang,Jaroslav Jelinek,Tatiana Karakasheva,Kathryn E Hamilton,Amanda B Muir,Marie-Pier Tétreault,Kelly A Whelan

Virologica Sinica : PubMed33534085

2021

ATP1B3 Restricts Hepatitis B Virus Replication Via Reducing the Expression of the Envelope Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zhang,Tianhang Zheng,Xiaolei Zhou,Hong Wang,Zhaolong Li,Chen Huan,Baisong Zheng,Wenyan Zhang

Journal of medical virology 92:201-209 PubMed31556466

2019

ATP1B3 cooperates with BST-2 to promote hepatitis B virus restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Baisong Zheng,Jun Zhang,Tianhang Zheng,Hong Wang,Zhaolong Li,Chen Huan,Shanshan Ning,Yingchao Wang,Wenyan Zhang

Epigenetics & chromatin 12:46 PubMed31331374

2019

Interplay between FACT subunit SPT16 and TRIM33 can remodel chromatin at macrophage distal regulatory elements.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Ferri,Vanessa Petit,Vilma Barroca,Paul-Henri Romeo

The Journal of biological chemistry 294:6375-6386 PubMed30792309

2019

A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na/K-ATPase in brain microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Hong Zhang,Dong-Xin Liu,Li Wang,Yu-Hua Li,Yan-Hua Wang,Hu Zhang,Zheng-Kang Su,Wen-Gang Fang,Xiao-Xue Qin,De-Shu Shang,Bo Li,Xiao-Ning Han,Wei-Dong Zhao,Yu-Hua Chen

JCI insight 3: PubMed30333310

2018

Increased expression of ATP12A proton pump in cystic fibrosis airways.

Applications

Unspecified application

Species

Unspecified reactive species

Paolo Scudieri,Ilaria Musante,Emanuela Caci,Arianna Venturini,Patrizia Morelli,Christine Walter,Davide Tosi,Alessandro Palleschi,Pablo Martin-Vasallo,Isabelle Sermet-Gaudelus,Gabrielle Planelles,Gilles Crambert,Luis Jv Galietta

Oncotarget 8:84285-84299 PubMed29137423

2017

Expression of the β3 subunit of Na/K-ATPase is increased in gastric cancer and regulates gastric cancer cell progression and prognosis via the PI3/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Ru Feng,Qian Xu,Feiyue Zhang,Tong Liu,Jiang Cao,Sujuan Fei

Channels (Austin, Tex.) 11:388-398 PubMed28636485

2017

Mineralocorticoids modulate the expression of the β-3 subunit of the Na, K-ATPase in the renal collecting duct.

Applications

Unspecified application

Species

Unspecified reactive species

Macarena Rojas,Pablo Díaz,Pablo León,Alexis A Gonzalez,Magdalena González,Víctor Barrientos,Nikolay B Pestov,Rodrigo Alzamora,Luis Michea

Molecular therapy : the journal of the American So 24:1760-1770 PubMed27434591

2016

Extracellular Antibody Drug Conjugates Exploiting the Proximity of Two Proteins.

Applications

Species

Unspecified reactive species

David J Marshall,Scott S Harried,John L Murphy,Chad A Hall,Mohammed S Shekhani,Christophe Pain,Conner A Lyons,Antonella Chillemi,Fabio Malavasi,Homer L Pearce,Jon S Thorson,James R Prudent

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP1B3 抗体 [EPR8981]

Anti-ATP1B3 antibody [EPR8981]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (AB137055)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (AB137055)

Western blot - Anti-ATP1B3 antibody [EPR8981] (AB137055)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (10)

Abcam product promise