Anti-ATP1B3 抗体 (ab67409)
Key features and details
- Mouse polyclonal to ATP1B3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ATP1B3 antibody
ATP1B3 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to ATP1B3 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mammals
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免疫原
Recombinant full length protein within Human ATP1B3 aa 1-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- ATP1B3 transfected 293T lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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ELISA pair antibody
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67409の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent dilution. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).
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| 特記事項 |
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WB
Use at an assay dependent dilution. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa). |
ターゲット情報
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機能
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known. -
配列類似性
Belongs to the X(+)/potassium ATPases subunit beta family. -
細胞内局在
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 483 Human
- Omim: 601867 Human
- SwissProt: P54709 Human
- Unigene: 477789 Human
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別名
- AT1B3_HUMAN antibody
- atp1b3 antibody
- ATPase Na+/K+ transporting beta 3 polypeptide antibody
see all
画像
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Western blot - Anti-ATP1B3 antibody (ab67409)All lanes : Anti-ATP1B3 antibody (ab67409) at 1/500 dilution
Lane 1 : ATP1B3 293T transfected lysate
Lane 2 : Non transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 31 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab67409 は 2 報の論文で使用されています。
- Zhang SH et al. A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. J Biol Chem 294:6375-6386 (2019). PubMed: 30792309
- Nishitsuji H et al. ATP1B3 Protein Modulates the Restriction of HIV-1 Production and Nuclear Factor ? Light Chain Enhancer of Activated B Cells (NF-?B) Activation by BST-2. J Biol Chem 291:4754-62 (2016). PubMed: 26694617
