AB315020

Anti-ATM (phospho S1987) 抗体 [EPR28058-71] - BSA and Azide free

Anti-ATM (phospho S1987) antibody [EPR28058-71] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ATM phospho S1987 antibody. Carrier free. Suitable for WB, Dot, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Transfected cell lysate - Mouse, Synthetic peptide - Mouse samples.

別名を表示する

Serine-protein kinase ATM, Ataxia telangiectasia mutated homolog, A-T mutated homolog, Atm

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-ATM (phospho S1987) antibody [EPR28058-71] - BSA and Azide free (AB315020)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATM (phospho S1987) antibody [EPR28058-71] - BSA and Azide free (AB315020)

This data was developed using ab315019, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MEF (mouse embryo fibroblast) cells labelling ATM (phospho S1987) with ab315019 at 1/100 (5.11 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing increased nuclear staining in MEF cells treated with UV-C 100 J/m2, then recovery for 4 hours. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Flow Cytometry (Intracellular) - Anti-ATM (phospho S1987) antibody [EPR28058-71] - BSA and Azide free (AB315020)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ATM (phospho S1987) antibody [EPR28058-71] - BSA and Azide free (AB315020)

This data was developed using ab315019, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MEF (mouse embryo fibroblast) treated with 100 J/m2 UV-C then recovery for 4h (Red) / Untreated MEF (Green) cells labelling ATM (phospho S1987) with ab315019 at 1/500 dilution (0.1 ug)/Red and Green compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Supplier Data

Western blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] - BSA and Azide free (AB315020)

This data was developed using ab315019, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the lower MW band at approximately 190 kDa is unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-ATM antibody [EPR20100] - (ab201022) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] (<a href='/products/primary-antibodies/atm-phospho-s1987-antibody-epr28058-71-ab315019'>ab315019</a>) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

NIH/3T3 treated with UV-C for 100J/cm2, then recovery 2 hours, whole cell lysate (untreated membrane) at 20 µg

Lane 3:

NIH/3T3 treated with UV-C for 100J/cm2, then recovery 2 hours, whole cell lysate (phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 349 kDa,124 kDa

false

Exposure time: 81s

Supplier Data

Western blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] - BSA and Azide free (AB315020)

This data was developed using ab315019, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the lower MW band at approximately 190 kDa is unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-ATM antibody [EPR20100] - (ab201022) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] (<a href='/products/primary-antibodies/atm-phospho-s1987-antibody-epr28058-71-ab315019'>ab315019</a>) at 1/1000 dilution

Lane 1:

Untreated MEF (mouse embryo fibroblast) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

MEF treated with UV-C for 100J/m2, then recovery 4 hours, whole cell lysate (untreated membrane) at 20 µg

Lane 3:

MEF treated with UV-C for 100J/m2, then recovery 4 hours, whole cell lysate (phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 349 kDa,124 kDa

false

Exposure time: 81s

Supplier Data

Western blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] - BSA and Azide free (AB315020)

This data was developed using ab315019, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the lower MW band at approximately 190 kDa and higher MW band at approximately 460 kDa are unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] (<a href='/products/primary-antibodies/atm-phospho-s1987-antibody-epr28058-71-ab315019'>ab315019</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse wild-type ATM expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse ATM (S1987A mutation) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 349 kDa,124 kDa

false

Exposure time: 180s

Supplier Data

Dot Blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] - BSA and Azide free (AB315020)

This data was developed using ab315019, the same antibody clone in a different buffer formulation.

Dot blot analysis of ATM (phospho S1987) using ab315019 at 1 : 1000 (0.511 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Exposure time : 180 seconds

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR28058-71

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse

アプリケーション

Dot, WB, Flow Cyt (Intra), ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

The antibody recognises Mouse ATM phosphorylated at Ser1987. This site is equivalent to phosphorylated Ser1981 in Human.

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Reactivity data

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製品の詳細

ab315020 is the carrier-free version of ab315019.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ATM also known as Ataxia Telangiectasia Mutated is a protein kinase with a molecular weight of approximately 370 kDa. ATM protein primarily resides in the cell nucleus and functions as a critical regulator of the cell cycle. It plays a significant role in the detection of DNA damage and initiation of repair processes. As part of its mechanical functions ATM phosphorylates serine and threonine residues on various substrates most notably in response to double-strand breaks in DNA. This activity is important for maintaining genomic stability.

Biological function summary

ATM acts as a coordinator in cellular response to DNA damage highly interacting with multiple components of the DNA repair machinery. It forms a complex with proteins like NBS1 and MRN complex facilitating repair by recruiting and activating other proteins involved in homologous recombination and non-homologous end joining pathways. ATM also modulates p53 activity a primary response factor in cellular stress management linking ATM to control of cell cycle arrest and apoptosis. This positions ATM as an integral part of maintaining cellular integrity in face of genomic insult.

Pathways

ATM integrates neatly within the DNA damage response and cell cycle control pathways. ATM's operative relationship with the MRN complex and its role in the PI3K-related protein kinase family helps initiate appropriate repair processes upon DNA damage detection. Additionally ATM regulates the activity of proteins such as Chk2 which further propagates signals to p53 influencing decisions between cell cycle arrest and apoptosis. These interactions link ATM closely to essential processes like DNA repair and cell survival highlighting its role in genomic maintenance.

ATM mutations or dysregulation leads to Ataxia Telangiectasia an autosomal recessive disorder characterized by neurodegeneration immune deficiencies and cancer predisposition. ATM dysfunction also connects to cancer development particularly breast cancer where it transmits signals involving BRCA1 contributing to DNA repair through homologous recombination. Understanding ATM dynamics and related pathways has important implications for developing therapeutic strategies to manage or mitigate effects associated with its dysfunction.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor (PubMed : 19047460). Recognizes the substrate consensus sequence [ST]-Q (PubMed : 19047460). Phosphorylates 'Ser-139' of histone variant H2AX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism (PubMed : 11571274). Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes (By similarity). After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele (PubMed : 19448632). Also involved in signal transduction and cell cycle control (By similarity). May function as a tumor suppressor (By similarity). Necessary for activation of ABL1 and SAPK (By similarity). Phosphorylates DYRK2, CHEK2, p53/TP53, FBXW7, FANCD2, NFKBIA, BRCA1, CREBBP/CBP, RBBP8/CTIP, MRE11, nibrin (NBN), RAD50, RAD17, PELI1, TERF1, UFL1, RAD9, UBQLN4 and DCLRE1C (By similarity). May play a role in vesicle and/or protein transport (By similarity). Could play a role in T-cell development, gonad and neurological function (By similarity). Binds DNA ends (By similarity). Plays a role in replication-dependent histone mRNA degradation (By similarity). Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation (By similarity). Phosphorylates ATF2 which stimulates its function in DNA damage response (By similarity). Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks (By similarity). Phosphorylates TTC5/STRAP at 'Ser-203' in the cytoplasm in response to DNA damage, which promotes TTC5/STRAP nuclear localization (By similarity). Also involved in pexophagy by mediating phosphorylation of PEX5 : translocated to peroxisomes in response to reactive oxygen species (ROS), and catalyzes phosphorylation of PEX5, promoting PEX5 ubiquitination and induction of pexophagy (By similarity).

See full target information Serine-protein kinase ATM phospho S1987

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATM (phospho S1987) 抗体 [EPR28058-71] - BSA and Azide free

Anti-ATM (phospho S1987) antibody [EPR28058-71] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-ATM (phospho S1987) antibody [EPR28058-71] - BSA and Azide free (AB315020)

Flow Cytometry (Intracellular) - Anti-ATM (phospho S1987) antibody [EPR28058-71] - BSA and Azide free (AB315020)

Western blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] - BSA and Azide free (AB315020)

Western blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] - BSA and Azide free (AB315020)

Western blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] - BSA and Azide free (AB315020)

Dot Blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] - BSA and Azide free (AB315020)

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise