Anti-ATM (phospho S1981) 抗体 [EP1890Y]
Anti-ATM (phospho S1981) antibody [EP1890Y]
- RabMAb
- Recombinant
- 詳細を見る
5
(18 Reviews)
|
(328 Publications)
Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292) is a rabbit monoclonal antibody detecting ATM in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, Dot Blot. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
- Over 320 publications
- Trusted since 2009
別名を表示する
Serine-protein kinase ATM, Ataxia telangiectasia mutated, A-T mutated, ATM
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling ATM (phospho S1981) with purified ab81292 at 1/70. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).
Negative control using PBS instead of primary antibody.
Counterstained with hematoxylin.
- WB
Unknown
Western blot - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292)
Blocking and dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292) at 1/50000 dilution
Lane 1:
HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate, untreated at 10 µg
Lane 2:
HEK-293 cell lysate, treated with Doxorubicin at 10 µg
Secondary
All lanes:
Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 351 kDa
Observed band size: 370 kDa
false
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial carcinoma tissue labeling ATM with unpurified ab81292.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292)
Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma) cells labeling ATM (phospho S1981) with purified ab81292 at 1/60 dilution (10μg/mL) (red).
Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human tonsil tissue labeling ATM with unpurified ab81292.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric carcinoma tissue labeling ATM with unpurified ab81292 at a 1/100 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling ATM with unpurified ab81292.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labeling ATM with unpurified ab81292.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IP
Unknown
Immunoprecipitation - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292)
ATM was immunoprecipitated from HEK-293 (Human embryonic kidney epithelial cell) treated with Doxorubicin whole cell lysate with ab81292 at 1/30 dilution (5 μg in 1 mg lysates). Western blot was performed from the immunoprecipitate using ab81292 at 1/2000 dilution. An anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.
Lane 1 : HEK-293 treated with Doxorubicin whole cell lysate 10 μg (Input).
Lane 2 : ab81292 IP in HEK-293 treated with Doxorubicin whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab81292 in HEK-293 treated with Doxorubicin whole cell lysate.
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 3 minutes.
All lanes:
Immunoprecipitation - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292)
Predicted band size: 351 kDa
false
- Dot
Unknown
Dot Blot - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292)
Dot blot analysis of ATM peptides using ab81292 at 1/000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody (ab97051) at 1/100,000 dilution.
Blocking and diluting buffer was 5% NFDM/TBST, exposure time 3 minutes.
Lane 1 : ATM (pS1981) phospho peptide
Lane 2 : ATM non-phospho peptide
Lane 3 : ATM (pS428) phospho peptide
関連する標識済み抗体及び組成の異なる製品 (10)
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Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free
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578 PE
PE Anti-ATM (phospho S1981) antibody [EP1890Y]
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660 APC
APC Anti-ATM (phospho S1981) antibody [EP1890Y]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-ATM (phospho S1981) antibody [EP1890Y]
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-ATM (phospho S1981) antibody [EP1890Y]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-ATM (phospho S1981) antibody [EP1890Y]
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-ATM (phospho S1981) antibody [EP1890Y]
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HRP Anti-ATM (phospho S1981) antibody [EP1890Y]
-
603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-ATM (phospho S1981) antibody [EP1890Y]
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775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-ATM (phospho S1981) antibody [EP1890Y]
Reactivity data
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製品の詳細
Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Dot blot, Flow Cyt (Intra), IHC-P, IP, WB in human samples.
Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292) has been cited over 328 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292) has 17 independent reviews from customers.
Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292) specifically detects ATM Phospho-S1981 (UniProt ID: Q13315; Molecular weight: 351kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EP1890Y - ab208775.
Antibody clone EP1890Y is also available pre-conjugated to a variety of labels for your convenience - PE, APC, HRP, Alkaline Phosphatase, Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 594, Alexa Fluor® 555 (ab305629, ab305630, ab305631, ab308654, ab309629, ab309995, ab310358, ab311880).
ATM is a significant target in oncology due to its role in DNA repair and maintaining genomic stability. Mutations in the ATM gene can lead to defective DNA repair, resulting in increased cancer risk and chemotherapy resistance.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
出荷温度及び保存条件
製品の状態
精製方法
バッファー組成
出荷温度
短期保存期間
短期保存温度
長期保存温度
分注に関する情報
保管に関する情報
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ATM acts as a coordinator in cellular response to DNA damage highly interacting with multiple components of the DNA repair machinery. It forms a complex with proteins like NBS1 and MRN complex facilitating repair by recruiting and activating other proteins involved in homologous recombination and non-homologous end joining pathways. ATM also modulates p53 activity a primary response factor in cellular stress management linking ATM to control of cell cycle arrest and apoptosis. This positions ATM as an integral part of maintaining cellular integrity in face of genomic insult.
Pathways
ATM integrates neatly within the DNA damage response and cell cycle control pathways. ATM's operative relationship with the MRN complex and its role in the PI3K-related protein kinase family helps initiate appropriate repair processes upon DNA damage detection. Additionally ATM regulates the activity of proteins such as Chk2 which further propagates signals to p53 influencing decisions between cell cycle arrest and apoptosis. These interactions link ATM closely to essential processes like DNA repair and cell survival highlighting its role in genomic maintenance.
製品プロトコール
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ターゲットの情報
文献 (328)
Recent publications for all applications. Explore the full list and refine your search
Science advances 9:eadi6681 PubMed38055811
2023
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Scientific reports 13:21211 PubMed38040841
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Cancer & metabolism 11:20 PubMed37932830
2023
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Nature communications 14:7069 PubMed37923710
2023
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Open medicine (Warsaw, Poland) 18:20230757 PubMed37840753
2023
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Signal transduction and targeted therapy 8:366 PubMed37743418
2023
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BMC cancer 23:835 PubMed37674118
2023
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Nature communications 14:5147 PubMed37620345
2023
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Cancers 15: PubMed37627223
2023
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Oncology reports 50: PubMed37477142
2023
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Abcam product promise
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