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AB322648

Anti-ATG14L 抗体 [EPR28551-44] - BSA and Azide free (Capture)

Anti-ATG14L antibody [EPR28551-44] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal ATG14L antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

別名を表示する

ATG14L, KIAA0831, ATG14, Beclin 1-associated autophagy-related key regulator, Barkor, Autophagy-related protein 14-like protein, Atg14L

3 Images
Sandwich ELISA - Anti-ATG14L antibody [EPR28551-44] - BSA and Azide free (Capture) (AB322648)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ATG14L antibody [EPR28551-44] - BSA and Azide free (Capture) (AB322648)

Sandwich ELISA of ab322647 with the capture antibody ab322648 dilution at 2 ug/mL and detector antibody ab322649 dilution at 0.5 ug/mL.

Sandwich ELISA - Anti-ATG14L antibody [EPR28551-44] - BSA and Azide free (Capture) (AB322648)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ATG14L antibody [EPR28551-44] - BSA and Azide free (Capture) (AB322648)

Interpolated concentrations of native ATG14L in human control wild type HeLa (human cervical adenocarcinoma epithelial cell) and ATG14 (ATG14L) knockout HeLa cell based on 250 ug/mL extract loads. The concentrations of ATG14L were measured in duplicate and interpolated from the ATG14L standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ATG14L concentration was determined to be 397.4 pg/mL in wild type HeLa extract (Human wild-type HeLa cell line ab255448) and 50.4 pg/mL in ATG14 (ATG14L) knockout HeLa extract (Human ATG14 (ATG14L) knockout HeLa cell line ab264684).

Sandwich ELISA - Anti-ATG14L antibody [EPR28551-44] - BSA and Azide free (Capture) (AB322648)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ATG14L antibody [EPR28551-44] - BSA and Azide free (Capture) (AB322648)

Interpolated concentration of native ATG14L was measured at different sample concentrations. Undiluted samples are as follows : HeLa cell extract (250 ug/mL) and HCT116 cell extract (400 ug/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR28551-44

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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製品の詳細

ab322648 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ATG14L. The recommended pair for sandwich ELISA is:

Capture: ab322648, Human ATG14L Capture Antibody (unconjugated)

Detector: ab322649, Human ATG14L Detector Antibody (unconjugated)

The reference range value is 15.63-2000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed : 18843052, PubMed : 19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed : 19270696, PubMed : 20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed : 20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed : 23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed : 23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed : 25686604, PubMed : 37632749). Modulates the hepatic lipid metabolism (By similarity).
See full target information ATG14
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Abcam product promise

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