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Anti-ATF2 抗体 (ab47476)

Reviews (1)Q&A (1)References (13)
Western blot - Anti-ATF2 antibody (ab47476)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody (ab47476)

Key features and details

  • Rabbit polyclonal to ATF2
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-ATF2 antibody [EPR22938-114] (ab239361)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-ATF2 antibody
    ATF2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to ATF2

  • 由来種

    Rabbit

  • 特異性

    ATF2 antibody detects endogenous levels of ATF2 protein around Threonine 71 or 53.

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human ATF2 aa 38-87.
    Database link: P15336

  • ポジティブ･コントロール

    • WB: Extracts from HeLa cells. IHC-P: Human breast carcinoma.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab47476の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/500 - 1/1000. Predicted molecular weight: 52 kDa.
IHC-P
Use at an assay dependent concentration.
特記事項
WB
1/500 - 1/1000. Predicted molecular weight: 52 kDa.
IHC-P
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.

  • 組織特異性

    Abundant expression seen in the brain.

  • 配列類似性

    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
    Contains 1 C2H2-type zinc finger.

  • 翻訳後修飾

    Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Activating transcription factor 2 antibody
      • Activating transcription factor 2 splice variant ATF2 var2 antibody
      • ATF 2 antibody
      • Atf-2 antibody
      • Atf2 antibody
      • ATF2 protein antibody
      • ATF2_HUMAN antibody
      • cAMP Response Element Binding Protein 2 antibody
      • cAMP response element binding protein CRE BP1 antibody
      • cAMP response element-binding protein CRE-BP1 antibody
      • cAMP responsive element binding protein 2, formerly antibody
      • cAMP-dependent transcription factor ATF-2 antibody
      • cAMP-responsive element-binding protein 2 antibody
      • CRE BP1 antibody
      • CRE-BP antibody
      • CREB 2 antibody
      • CREB-2 antibody
      • CREB2 antibody
      • CREBP1 antibody
      • Cyclic AMP dependent transcription factor ATF 2 antibody
      • Cyclic AMP-dependent transcription factor ATF-2 antibody
      • Cyclic AMP-responsive element-binding protein 2 antibody
      • D130078H02Rik antibody
      • D18875 antibody
      • HB 16 antibody
      • HB16 antibody
      • Histone acetyltransferase ATF2 antibody
      • MGC105211 antibody
      • MGC105222 antibody
      • MGC111558 antibody
      • MGC142504 antibody
      • mXBP antibody
      • MXBP protein antibody
      • Tg(Gzma-Klra1)7Wum antibody
      • TREB 7 antibody
      • TREB7 antibody
      see all

    画像

    • Western blot - Anti-ATF2 antibody (ab47476)
      Western blot - Anti-ATF2 antibody (ab47476)
      All lanes : Anti-ATF2 antibody (ab47476) at 1/500 dilution

      Lane 1 : extracts from HeLa cells.
      Lane 2 : extracts from HeLa cells preincubated with immunizing peptide

      Predicted band size: 52 kDa
      Observed band size: 52 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody (ab47476)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody (ab47476)

      Immunohistochemical analysis of paraffin-embedded human breast carcinoma. Left: Using ATF2 antibody; Right: The same antibody preincubated with synthesized peptide.

    参考文献 (13)

    ab47476 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab47476 は 13 報の論文で使用されています。

    • Zhao K  et al. microRNA-125b and its downstream Smurf1/KLF2/ATF2 axis as important promoters on neurological function recovery in rats with spinal cord injury. J Cell Mol Med N/A:N/A (2021). PubMed: 33951295
    • Hu X  et al. Ubiquitin fold modifier 1 activates NF-?B pathway by down-regulating LZAP expression in the macrophage of diabetic mouse model. Biosci Rep 40:N/A (2020). PubMed: 31829413
    • Han F  et al. Exosomal microRNA-26b-5p down-regulates ATF2 to enhance radiosensitivity of lung adenocarcinoma cells. J Cell Mol Med 24:7730-7742 (2020). PubMed: 32476275
    • Gao T  et al. KIF18B promotes tumor progression in osteosarcoma by activating ß-catenin. Cancer Biol Med 17:371-386 (2020). PubMed: 32587775
    • Li P  et al. Inhibition of long noncoding RNA HIF1A-AS2 confers protection against atherosclerosis via ATF2 downregulation. J Adv Res 26:123-135 (2020). PubMed: 33133688
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-ATF2 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (endothelial)
    Loading amount
    5000 cells
    Specification
    endothelial
    Treatment
    different shear stress exposure for 24 hours
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 01 2010

    Question

    Hello , Here is the complain of two antibodies .

    1) Abcam catalogue no:

    Ab31419, Ab47476

    2) a) Abcam order number (not required):

    1148646


    b) Abcam product lot number:

    c-Jun> GR50836-9, ATF-2> GR72060-1

    3) Description of the problem (high background, wrong band size, more bands, no band etc):

    No bands

    4) Antibody storage conditions (temperature/reconstitution etc):

    -20°C


    5) a)Sample (Species):


    c-Jun and ATF2 from HeLa


    b) Type of sample (Cell extract/Nuclear extract/Purified protein/Recombinant protein etc).:


    Whole cell extract

    6) a) Sample preparation:

    Lysis buffer



    RIPA





    Protease inhibitors



    AEBSF, 2 mM
    Aprotinin, 0.3 μM
    Bestatin, 130 μM
    EDTA, 1 mM
    E-64, 14 μM
    Leupeptin, 1 μM

    PMSF, 20 mM





    Phosphatase inhibitors



    -





    Reducing agent



    SDS and β-ME





    Boiling for ≥5 min?



    5 minutes







    b) Amount of protein loaded:







    Protein loaded ug/lane or cells/lane



    5 μg/lane







    7) a) Electrophoresis/Gel conditions:







    Reducing or Non Reducing gel



    Reducing





    Percentage of gel



    15%





    Volts applied



    75





    Time applied



    3 hours

















    b) Transfer or blocking conditions:







    Type of membrane



    PVDF





    Protein transfer verified



    Ponceau, β-actin





    Blocking agent and concentration



    3% BSA





    Blocking time



    30 minutes





    Blocking temperature



    25°C

















    8) Primary antibody (If more than one was used, describe in “additional notes”):









    Concentration or dilution



    1/1000





    Diluent buffer



    1% BSA + 0.05% Tween-20 in 1X PBS





    Incubation time



    2 hours





    Incubation temperature



    25°C





    Washing: Buffer Used



    0.05% Tween-20 in 1X PBS





    Number of washes



    3







    9) Secondary antibody:









    Species



    Goat





    Reacts against



    Rabbit





    Concentration or dilution



    1/10,000





    Diluent buffer



    1% BSA + 0.05% Tween-20 in 1X PBS





    Incubation time



    2 hours





    Incubation temperature:



    25°C





    Fluorochrome or enzyme conjugate



    HRP





    Washing: Buffer Used



    0.05% Tween-20 in 1X PBS





    Number of washes



    3







    10) Detection method (ECL, ECL plus, etc.):









    ECL





    11) Positive and negative controls used:









    Positive control



    β-actin





    Negative control











    12) a) How many times you have run this staining?







    Twice







    b) Do you obtain the same results every time?







    No







    C) What steps have you altered to try and optimize the use of this antibody?







    Reducing blocking, primary and secondary incubation times while attempting the second time. Washing was made slightly rigorous as compared to he first time. Performed a Dot blot to check for performance of secondary antibody and it works fine. Increased amount of loaded protein to 10 μg/lane.







    Regards

    Read More
    Answer

    Thank you for your enquiry regarding ab31419 and ab47476; and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with these two antibodies.
    I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.
    After reading through the detailed protocol you kindly forwarded to Abcam, I would like to make the following comments/suggestions:
    1) The loaded amount of protein seems to be a bit low: 5 μg/lane, normally we would suggest loading at least 25-30 μg/lane protein per lane.
    2) Since c-Jun and ATF2 are both mainly present in the nuclei, it would be worth preparing nuclear fraction (rather than whole cell lysate) to enrich the target proteins in the sample if it is possible and apply them for WB.
    3) Customer may also need to test different dilutions (1/500 or even 1/250) to make sure that the experiment is carried out under the optimal conditions. This is even more important since only 5 μg instead of 30 μg lysate was loaded.
    I hope this will be useful for you. Should you still have any problem with this antibody after following these suggestions, then please do not hesitate to contact our Technical Department again.

    Read More

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