Anti-ATF2 抗体 (ab31483)
Key features and details
- Rabbit polyclonal to ATF2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ATF2 antibody
ATF2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ATF2 -
由来種
Rabbit -
特異性
This antibody detects endogenous levels of ATF2 protein around Threonine 73 or 55. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide around the phosphorylation site of Threonine 73 or 55 (Human)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31483の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
|
| IHC-P |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex. -
組織特異性
Abundant expression seen in the brain. -
配列類似性
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger. -
翻訳後修飾
Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1386 Human
- Omim: 123811 Human
- SwissProt: P15336 Human
- Unigene: 592510 Human
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別名
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody (ab31483)Ab31483, at a dilution of 1/50, staining ATF2 in paraffin embedded breast carcinoma tissue byImmunohistochemistry.
Left image: Using ab31483.
Right image: The same antibody preincubated with synthesized peptide. -
Western blot - Anti-ATF2 antibody (ab31483)All lanes : Anti-ATF2 antibody (ab31483) at 1/500 dilution
Lane 1 : Extracts from LOVO cells (5-30ug) treated with ab31483.
Lane 2 : Extracts from LOVO cells (5-30ug) treated using the same antibody preincubated with
synthesized peptide.
Secondary
All lanes : goat-anti-rabbit IgG-AP-conjugate.
Predicted band size: 54 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab31483 は論文での使用が確認できていません。