Anti-ATF2 抗体 (ab245407)
Key features and details
- Rabbit polyclonal to ATF2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ATF2 antibody
ATF2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ATF2 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human ATF2 aa 437-487. The exact sequence is proprietary. AAY17203.1
Database link: P15336 -
ポジティブ･コントロール
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245407 was affinity purified using an epitope specific to ATF2 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245407の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 55 kDa.
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| 特記事項 |
|---|
|
IP
Use at 2-5 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 55 kDa. |
ターゲット情報
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機能
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex. -
組織特異性
Abundant expression seen in the brain. -
配列類似性
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger. -
翻訳後修飾
Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1386 Human
- Omim: 123811 Human
- SwissProt: P15336 Human
- Unigene: 592510 Human
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別名
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
see all
画像
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Western blot - Anti-ATF2 antibody (ab245407)All lanes : Anti-ATF2 antibody (ab245407) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 55 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-ATF2 antibody (ab245407)ATF2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245407 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245407 at 1 µg/ml.
Lane 1: ab245407 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245407 は論文での使用が確認できていません。