Anti-Ataxin 7 抗体 (ab11434)
Key features and details
- Rabbit polyclonal to Ataxin 7
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Ataxin 7 antibody
Ataxin 7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ataxin 7 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide corresponding to Human Ataxin 7 aa 1-17.
Sequence:MSERAADDVRGEPRRAA
(Peptide available asab39761) -
ポジティブ･コントロール
- ICC/IF: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate ; U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate WB: Transfected COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11434の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 96 kDa.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 96 kDa. |
ターゲット情報
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機能
Acts as component of the STAGA transcription coactivator-HAT complex. Mediates the interaction of STAGA complex with the CRX and is involved in CRX-dependent gene activation. Necessary for microtubule cytoskeleton stabilization. -
組織特異性
Isoform a and isoform b are expressed in CNS, but isoform a is expressed predominantly in the peripherical tissues. Isoform b is also highly expressed in the frontal lobe, skeletal muscle and spinal cord and is expressed at a lower level in the lung, lymphoblast and intestine. -
関連疾患
Spinocerebellar ataxia 7 -
配列類似性
Belongs to the ataxin-7 family.
Contains 1 SCA7 domain. -
翻訳後修飾
Proteolytically cleaved. The cleavage may be involved in SCA7 degeneration: the isoform fragments may exert distinct toxic influences that could contribute to selective neurodegeneration.
Sumoylation decreases the aggregation propensity and cellular toxicity of forms with an expanded poly-Gln region but has no effect on subcellular location or interaction with components of the STAGA complex. -
細胞内局在
Cytoplasm and Nucleus. Nucleus, nucleolus. Nucleus matrix. Cytoplasm, cytoskeleton. In addition to a diffuse distribution throughout the nucleus, it is associated with the nuclear matrix and the nucleolus. It is able to shuttle between the nucleus and cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 6314 Human
- Entrez Gene: 246103 Mouse
- Omim: 164500 Human
- Omim: 607640 Human
- SwissProt: O15265 Human
- SwissProt: Q8R4I1 Mouse
- Unigene: 476595 Human
- Unigene: 133625 Mouse
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別名
- ADCAII antibody
- Ataxin-7 antibody
- ATX7_HUMAN antibody
see all
画像
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Western blot - Anti-Ataxin 7 antibody (ab11434)Western blot of Ataxin 7 in transfected COS-1 cell lysate using ab11434.
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Immunocytochemistry/ Immunofluorescence - Anti-Ataxin 7 antibody (ab11434)ICC/IF image of ab11434 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11434, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-Ataxin 7 antibody (ab11434)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton™ X-100 permeabilized, 70% confluent log phase U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells, treated with 100 ng of Nocodazole for 16 hours. Labelling Ataxin 7 with ab11434 at 2 µg/mL and incubated for 3 hours at room temperature and then labelled with Goat anti-Rabbit IgG secondary antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Green). Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (Blue). F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin at a dilution of 1/300 (Red). Panel e is untreated cell with nuclear localization. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab11434 は 3 報の論文で使用されています。
- Nath S et al. Functional characterization of variants of unknown significance in a spinocerebellar ataxia patient using an unsupervised machine learning pipeline. Hum Genome Var 9:10 (2022). PubMed: 35422034
- Suárez-Sánchez R et al. RNA Foci Formation in a Retinal Glial Model for Spinocerebellar Ataxia Type 7. Life (Basel) 13:N/A (2022). PubMed: 36675972
- Datson NA et al. The expanded CAG repeat in the huntingtin gene as target for therapeutic RNA modulation throughout the HD mouse brain. PLoS One 12:e0171127 (2017). PubMed: 28182673