Anti-Ataxin 1 (phospho S776) 抗体 (ab63376)
Key features and details
- Rabbit polyclonal to Ataxin 1 (phospho S776)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Ataxin 1 (phospho S776) antibody
Ataxin 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ataxin 1 (phospho S776) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Ataxin 1 aa 700-800 (phospho S776).
Database link: P54253 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63376の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa). |
ターゲット情報
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機能
Binds RNA in vitro. May be involved in RNA metabolism. The expansion of the polyglutamine tract may alter this function. -
組織特異性
Widely expressed throughout the body. -
関連疾患
Defects in ATXN1 are the cause of spinocerebellar ataxia type 1 (SCA1) [MIM:164400]; also known as olivopontocerebellar atrophy I (OPCA I or OPCA1). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to cerebellum degeneration with variable involvement of the brainstem and spinal cord. SCA1 belongs to the autosomal dominant cerebellar ataxias type I (ADCA I) which are characterized by cerebellar ataxia in combination with additional clinical features like optic atrophy, ophthalmoplegia, bulbar and extrapyramidal signs, peripheral neuropathy and dementia. SCA1 is caused by expansion of a CAG repeat in the coding region of ATXN1. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. -
配列類似性
Belongs to the ATXN1 family.
Contains 1 AXH domain. -
ドメイン
The AXH domain is required for interaction with CIC. -
翻訳後修飾
Phosphorylation at Ser-775 increases the pathogenicity of proteins with an expanded polyglutamine tract.
Sumoylation is dependent on nuclear localization and phosphorylation at Ser-775. It is reduced in the presence of an expanded polyglutamine tract. -
細胞内局在
Cytoplasm. Nucleus. Colocalizes with USP7 in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6310 Human
- Omim: 601556 Human
- SwissProt: P54253 Human
- Unigene: 434961 Human
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別名
- alternative ataxin1 antibody
- Ataxin-1 antibody
- ATX1 antibody
see all
画像
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Western blot - Anti-Ataxin 1 (phospho S776) antibody (ab63376)All lanes : Anti-Ataxin 1 (phospho S776) antibody (ab63376) at 1/500 dilution
Lane 1 : HepG2 cell extract treated with Adriamycin (0.5 micromoles, 5hours)
Lane 2 : HepG2 cell extract treated with Adriamycin (0.5 micromoles, 5hours) with immunizing phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 87 kDa
Observed band size: 87 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab63376 は 2 報の論文で使用されています。
- Brown AS et al. MTSS1/Src family kinase dysregulation underlies multiple inherited ataxias. Proc Natl Acad Sci U S A 115:E12407-E12416 (2018). PubMed: 30530649
- Hearst SM et al. Dopamine D2 receptor signaling modulates mutant ataxin-1 S776 phosphorylation and aggregation. J Neurochem 114:706-16 (2010). ICC/IF ; Human . PubMed: 20477910