Anti-Ataxin 1 抗体 [N76/8] (ab186265)
Key features and details
- Mouse monoclonal [N76/8] to Ataxin 1
- Suitable for: WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
製品の概要
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製品名
Anti-Ataxin 1 antibody [N76/8]
Ataxin 1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [N76/8] to Ataxin 1 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human, Recombinant fragment
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide corresponding to Mouse Ataxin 1 aa 150-250.
Database link: P54254 -
ポジティブ・コントロール
- Rat brain lysate.
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特記事項
The clone number has been updated from S76-8 to N76/8, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
N76/8 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186265の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/1000. Predicted molecular weight: 84 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 84 kDa. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Binds RNA in vitro. May be involved in RNA metabolism. The expansion of the polyglutamine tract may alter this function. -
組織特異性
Widely expressed throughout the body. -
関連疾患
Defects in ATXN1 are the cause of spinocerebellar ataxia type 1 (SCA1) [MIM:164400]; also known as olivopontocerebellar atrophy I (OPCA I or OPCA1). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to cerebellum degeneration with variable involvement of the brainstem and spinal cord. SCA1 belongs to the autosomal dominant cerebellar ataxias type I (ADCA I) which are characterized by cerebellar ataxia in combination with additional clinical features like optic atrophy, ophthalmoplegia, bulbar and extrapyramidal signs, peripheral neuropathy and dementia. SCA1 is caused by expansion of a CAG repeat in the coding region of ATXN1. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. -
配列類似性
Belongs to the ATXN1 family.
Contains 1 AXH domain. -
ドメイン
The AXH domain is required for interaction with CIC. -
翻訳後修飾
Phosphorylation at Ser-775 increases the pathogenicity of proteins with an expanded polyglutamine tract.
Sumoylation is dependent on nuclear localization and phosphorylation at Ser-775. It is reduced in the presence of an expanded polyglutamine tract. -
細胞内局在
Cytoplasm. Nucleus. Colocalizes with USP7 in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6310 Human
- Entrez Gene: 20238 Mouse
- Omim: 601556 Human
- SwissProt: P54253 Human
- SwissProt: P54254 Mouse
- Unigene: 434961 Human
- Unigene: 342683 Mouse
- Unigene: 342686 Mouse
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別名
- alternative ataxin1 antibody
- Ataxin-1 antibody
- ATX1 antibody
see all
画像
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Anti-Ataxin 1 antibody [N76/8] (ab186265) at 1/200 dilution + Monkey COS-1 cells transfected with Ataxin- 1 at 15 µg
Secondary
Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT
Predicted band size: 84 kDa -
Immunocytochemistry/Immunofluorescence analysis of 4% Formaldehyde-fixed human SK-N-BE Cells (Human Neuroblastoma cells) labeling Ataxin 1 (green) using ab186265 at 1/100 dilution P for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab186265 は論文での使用が確認できていません。