Anti-Asporin 抗体 (ab31303)
Key features and details
- Goat polyclonal to Asporin
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Asporin antibody
Asporin 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Asporin -
由来種
Goat -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Asporin aa 150-250 (internal sequence).
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ポジティブ･コントロール
- WB: Human tonsil, human uterus, mouse and rat skeletal muscle tissue lysates. ICC: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31303の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
1 hour primary incubation is recommended for this product. |
| ICC |
Use at an assay dependent concentration.
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| 特記事項 |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa). 1 hour primary incubation is recommended for this product. |
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ICC
Use at an assay dependent concentration. |
ターゲット情報
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機能
Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins (By similarity). Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity. -
組織特異性
Higher levels in osteoarthritic articular cartilage, aorta, uterus. Moderate expression in small intestine, heart, liver, bladder, ovary, stomach, and in the adrenal, thyroid, and mammary glands. Low expression in trachea, bone marrow, and lung. Co-localizes with TGFB1 in chondrocytes within osteoarthritic (OA) lesions of articular cartilage. -
関連疾患
Genetic variations in ASPN are associated with susceptibility to osteoarthritis type 3 (OS3) [MIM:607850]; also known as osteoarthritis of knee/hip. Osteoarthritis is a degenerative disease of the joints characterized by degradation of the hyaline articular cartilage and remodeling of the subchondral bone with sclerosis. Clinical symptoms include pain and joint stiffness often leading to significant disability and joint replacement. Note=Susceptibility to osteoarthritis is conferred by a triplet repeat expansion polymorphism. ASPN allele having 14 aspartic acid repeats in the N-terminal region of the protein (D14), is overrepresented relative to the common allele having 13 aspartic acid repeats (D13). The frequency of the D14 allele increases with disease severity. The D14 allele is also overrepresented in individuals with hip osteoarthritis.
Defects in ASPN are a cause of susceptibility to intervertebral disk disease (IDD) [MIM:603932]. A common musculo-skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Note=Susceptibility to intervertebral disk disease, particularly lumbar disk degeneration, is conferred by a triplet repeat expansion polymorphism. ASPN allele having 14 aspartic acid repeats in the N-terminal region of the protein (D14), is associated with the disorder in some populations (PubMed:18304494). -
配列類似性
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
ドメイン
The LRR 5 repeat can inhibit BMP2-induced cytodifferentiation and may be involved in the interaction with BMP2 (By similarity). The repeats LRR 10, LRR 11 and LRR 12 are involved in binding type I collagen. The poly-Asp region is involved in binding calcium. -
翻訳後修飾
There is no serine/glycine dipeptide sequence expected for the attachment of O-linked glycosaminoglycans and this is probably not a proteoglycan. The O-linked polysaccharide on 54-Ser is probably the mucin type linked to GalNAc.
The N-linked glycan at Asn-282 is composed of variable structures of GlcNAc, mannose, fucose, HexNAc and hexose. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 54829 Human
- Entrez Gene: 66695 Mouse
- Entrez Gene: 306805 Rat
- Omim: 608135 Human
- SwissProt: Q9BXN1 Human
- SwissProt: Q99MQ4 Mouse
- Unigene: 435655 Human
- Unigene: 383216 Mouse
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別名
- ASPN antibody
- ASPN protein antibody
- ASPN_HUMAN antibody
see all
画像
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Western blot - Anti-Asporin antibody (ab31303)Lanes 1 & 3 & 5 : Anti-Asporin antibody (ab31303) at 0.1 µg/ml
Lane 2 : Anti-Asporin antibody (ab31303) at 0.3 µg/ml
Lane 4 : Anti-Asporin antibody (ab31303) at 1 µg/ml
Lane 1 : Human tonsil tissue lysate
Lane 2 : Human uterus tissue lysate
Lane 3 : Mouse skeletal muscle tissue lysate
Lane 4 : Rat skeletal muscle tissue lysate
Lane 5 : Human cerebellum tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 43 kDa35µg protein in RIPA buffer. Detected by chemiluminescence.
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Immunocytochemistry - Anti-Asporin antibody (ab31303)Immunocytochemistry analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Incubated with ab31303 for 1 hour (10µg/ml) followed by Alexa Fluor® 488 secondary antibody (4µg/ml), showing nuclear membrane staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor® 488 secondary antibody (4µg/ml).
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Western blot - Anti-Asporin antibody (ab31303)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Asporin antibody (ab31303) at 1/1000 dilution
All lanes : Human osteosarcoma cell line whole lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-goat IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocked with 5% milk for 1 hour at 27°C.
Incubated with the primary antibody diluted in blocking buffer for 12 hours at 4°C.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab31303 は 10 報の論文で使用されています。
- Zhou G et al. ASPN Synergizes with HAPLN1 to Inhibit the Osteogenic Differentiation of Bone Marrow Mesenchymal Stromal Cells and Extracellular Matrix Mineralization of Osteoblasts. Orthop Surg 15:2423-2434 (2023). PubMed: 37427673
- Chen Y et al. Single-cell RNA landscape of the osteoimmunology microenvironment in periodontitis. Theranostics 12:1074-1096 (2022). PubMed: 35154475
- Yin L et al. Identification of a five m6A-relevant mRNAs signature and risk score for the prognostication of gastric cancer. J Gastrointest Oncol 13:2234-2248 (2022). PubMed: 36388685
- Huang C et al. Asporin, an extracellular matrix protein, is a beneficial regulator of cardiac remodeling. Matrix Biol 110:40-59 (2022). PubMed: 35470068
- Wietecha MS et al. Activin-mediated alterations of the fibroblast transcriptome and matrisome control the biomechanical properties of skin wounds. Nat Commun 11:2604 (2020). PubMed: 32451392
- Wang Y et al. Low SCRIB expression in fibroblasts promotes invasion of lung cancer cells. Life Sci 256:117955 (2020). PubMed: 32534038
- Maccarana M et al. Asporin-deficient mice have tougher skin and altered skin glycosaminoglycan content and structure. PLoS One 12:e0184028 (2017). PubMed: 28859141
- Hurng JM et al. Discontinuities in the human bone-PDL-cementum complex. Biomaterials 32:7106-17 (2011). ICC/IF ; Human . PubMed: 21774982
- Duval E et al. Asporin expression is highly regulated in human chondrocytes. Mol Med 17:816-23 (2011). PubMed: 21528154
- Ho SP et al. The biomechanical characteristics of the bone-periodontal ligament-cementum complex. Biomaterials 31:6635-46 (2010). IHC-P ; Human . PubMed: 20541802