Anti-Aspartate beta hydroxylase 抗体 [EPR10351] (ab172475)
![Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.co.jp/ps/products/172/ab172475/Images/ab172475-194726-anti-aspartate-beta-hydroxylase-antibody-epr10351-western-blot.jpg "Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10351] to Aspartate beta hydroxylase
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Aspartate beta hydroxylase antibody [EPR10351]
Aspartate beta hydroxylase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10351] to Aspartate beta hydroxylase -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
適用なし: IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Aspartate beta hydroxylase aa 250-350 (Cysteine residue). The exact sequence is proprietary.
Database link: Q12797 -
ポジティブ･コントロール
- HeLa, A549 and HepG2 whole cell lysate (ab7900); Human colon carcinoma and pancreas tissues; HeLa cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR10351 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab172475の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/10000 - 1/50000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 86 kDa).
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/250 - 1/500.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 86 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/250 - 1/500. |
ターゲット情報
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関連性
ASPH is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression. Variation among these transcripts impacts their functions which involve roles in the calcium storage and release process in the endoplasmic and sarcoplasmic reticulum as well as hydroxylation of aspartic acid and asparagine in epidermal growth factor like domains of various proteins. -
細胞内局在
Endoplasmic reticulum; endoplasmic reticulum membrane; Single-pass type II membrane protein. -
参照データベース
- Entrez Gene: 444 Human
- Omim: 600582 Human
- SwissProt: Q12797 Human
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別名
- A beta H J J antibody
- AAH antibody
- ASP beta hydroxylase antibody
see all
画像
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Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)All lanes : Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 86 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.co.jp/ps/products/172/ab172475/Images/ab172475-194728-anti-aspartate-beta-hydroxylase-antibody-epr10351-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Aspartate beta hydroxylase with ab172475 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.co.jp/ps/products/172/ab172475/Images/ab172475-194727-anti-aspartate-beta-hydroxylase-antibody-epr10351-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling Aspartate beta hydroxylase with ab172475 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.co.jp/ps/products/172/ab172475/Images/ab172475-194729-anti-aspartate-beta-hydroxylase-antibody-epr10351-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Immunofluorescent analysis of HeLa cells labeling Aspartate beta hydroxylase with ab172475 at 1/250 dilution (red). DAPI nuclear staining (blue).
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![Flow Cytometry (Intracellular) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.co.jp/ps/products/172/ab172475/Images/ab172475-194730-anti-aspartate-beta-hydroxylase-antibody-epr10351-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Intracellular flow cytometric analysis of permeabilized HeLa cells labeling Aspartate beta hydroxylasewith ab172475at 1/10 dilution (red) compared to a Rabbit IgG (negative) (green).
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![Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.co.jp/ps/products/172/ab172475/Images/ab172475-6-benefits-of-recombinant-antibodies.png)
Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab172475 は 1 報の論文で使用されています。
- Chen X et al. miR-135a acts as a tumor suppressor by targeting ASPH in endometrial cancer. Int J Clin Exp Pathol 12:3384-3389 (2019). PubMed: 31934181