Anti-Aspartate Aminotransferase + FABP-1 抗体 [EPR12145] (ab170950)

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Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12145] to Aspartate Aminotransferase + FABP-1
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

製品名

Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]
Aspartate Aminotransferase+FABP-1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR12145] to Aspartate Aminotransferase + FABP-1
由来種

Rabbit
特異性

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
アプリケーション

適用あり: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- HepG2; MCF-7, HT-29 and K562 cell lysates; Human hepatocellular carcinoma tissue; HepG2 and K562 cells. Mouse brain, rat brain and rat heart lysates. Human glioma tissue, K562.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR12145
アイソタイプ

IgG
研究分野

Alternative Versions
Assay kits
- Aspartate Aminotransferase Activity Assay Kit (ab105135)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- K-562 whole cell lysate (ab7911)
Recombinant Protein
- Recombinant mouse Aspartate Aminotransferase protein (His tag) (ab201889)
Related Products
- Recombinant Human Aspartate Aminotransferase protein (ab99147)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab170950の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB		1/3000. Predicted molecular weight: 46 kDa.
ICC/IF		1/500.
IHC-P		1/170. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

特記事項
Flow Cyt (Intra) 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/3000. Predicted molecular weight: 46 kDa.
ICC/IF 1/500.
IHC-P 1/170. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

細胞内局在

Aspartate Aminotransferase: Cytoplasm. FABP-1: Mitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane.
参照データベース
- Entrez Gene: 2805 Human
- Entrez Gene: 2806 Human
- Entrez Gene: 14718 Mouse
- Entrez Gene: 14719 Mouse
- Entrez Gene: 24401 Rat
- Entrez Gene: 25721 Rat
- Omim: 138150 Human
- Omim: 138180 Human
- SwissProt: P00505 Human
- SwissProt: P17174 Human
- SwissProt: P05201 Mouse
- SwissProt: P05202 Mouse
- SwissProt: P00507 Rat
- SwissProt: P13221 Rat
- Unigene: 500756 Human
- Unigene: 599470 Human
- Unigene: 19039 Mouse
- Unigene: 230169 Mouse
- Unigene: 5819 Rat
- Unigene: 98650 Rat
see all

Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

All lanes : purified at 1/3000 dilution

Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lane 4 : Mouse brain lysates
Lane 5 : Mouse heart lysates
Lane 6 : Rat brain lysates
Lane 7 : Rat heart lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 46 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950) at 1/1000 dilution + Recombinant human FABP-1 protein (ab206788) at 0.015 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 46 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds
Immunocytochemistry/ Immunofluorescence - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) cells labelling Aspartate Aminotransferase + FABP-1 with purified ab170950 at 1/120. Cells were fixed with 100% methanol. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
Flow Cytometry (Intracellular) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Aspartate Aminotransferase + FABP-1 with purified ab170950 at 1/20 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling Aspartate Aminotransferase + FABP-1 with Purified ab170950 at 1:170 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/50 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

All lanes : Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950) at 1/1000 dilution (unpurified)

Lane 1 : HepG2 cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 46 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

Immunofluorescent analysis of HepG2 cells labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/50 dilution (green). DAPI nuclear staining (blue).
Flow Cytometry (Intracellular) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

Intracellular flow cytometric analysis of permeabilized K562 cells labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/10 dilution (red) or a rabbit IgG negative (green).
Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab170950 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab170950 は 7 報の論文で使用されています。

Kremer DM et al. GOT1 inhibition promotes pancreatic cancer cell death by ferroptosis. Nat Commun 12:4860 (2021). PubMed: 34381026
Yuan C et al. Surgical Preparation Reduces Hydrogen Sulfide Released from Human Saphenous Veins in Coronary Artery Bypass Grafting. J Cardiovasc Transl Res 13:181-190 (2020). PubMed: 31712976
van Gastel N et al. Induction of a Timed Metabolic Collapse to Overcome Cancer Chemoresistance. Cell Metab 32:391-403.e6 (2020). PubMed: 32763164
Lee JS et al. Loss of SLC25A11 causes suppression of NSCLC and melanoma tumor formation. EBioMedicine 40:184-197 (2019). PubMed: 30686754
Yuan C et al. Hydrogen sulfide-mediated endothelial function and the interaction with eNOS and PDE5A activity in human internal mammary arteries. J Int Med Res 47:3778-3791 (2019). PubMed: 31155983
Tajan M et al. A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3. Cell Metab 28:721-736.e6 (2018). PubMed: 30122553
Zhu Y et al. A Critical Role of Glutamine and Asparagine ?-Nitrogen in Nucleotide Biosynthesis in Cancer Cells Hijacked by an Oncogenic Virus. MBio 8:N/A (2017). PubMed: 28811348

レビューと Q&A

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レビューを送る質問を送る

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Brain)

Permeabilization

Yes - Triton X-100

Specification

Brain

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Jul 11 2016

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Assay kits

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

細胞内局在

参照データベース

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (7)

レビューと Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Aspartate Aminotransferase antibody [EPR12145]