AB40850

Anti-Asparagine synthetase 抗体 [EP282Y]

Name: Anti-Asparagine synthetase 抗体 [EP282Y] (ab40850) | Abcam
Brand: Abcam Limited
SKU: AB40850
Rating: 1 (1 reviews)

Anti-Asparagine synthetase antibody [EP282Y]

RabMAb
Recombinant
詳細を見る

(1 Review)

(6 Publications)

Rabbit Recombinant Monoclonal Asparagine synthetase antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.

別名を表示する

TS11, ASNS, Asparagine synthetase [glutamine-hydrolyzing], Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase

1 Images

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Western blot - Anti-Asparagine synthetase antibody [EP282Y] (AB40850)

All lanes:

Western blot - Anti-Asparagine synthetase antibody [EP282Y] (ab40850) at 1/2000 dilution

All lanes:

K562 cell lysate at 10 µg

Predicted band size: 64 kDa

Observed band size: 60 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP282Y

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Asparagine synthetase (ASNS) is an enzyme that catalyzes the conversion of aspartate and glutamine to asparagine and glutamate. This enzyme is also known by the alternate name ASPG. Asparagine synthetase weighs approximately 64 kDa and it is found in many tissues including the liver and pancreas. The presence of ASNS is important for the synthesis of asparagine an amino acid necessary for protein and nucleotide synthesis.

Biological function summary

ASNS drives the synthesis of asparagine through a mechanism involving the ATP-dependent conversion of substrates. It does not function as part of a larger enzyme complex but operates independently to fulfill its role. In cells asparagine produced serves as a critical building block supporting protein biosynthesis and cell proliferation particularly in rapidly dividing cells such as cancer cells.

Pathways

Asparagine synthetase plays a central role in the asparagine biosynthetic pathway. This enzyme is key for maintaining cellular amino acid homeostasis and is linked to the mTOR pathway which regulates cell growth and metabolism. Additionally ASNS interacts with proteins like glutaminase which supplies one of its essential substrates glutamine.

ASNS has a significant correlation with acute lymphoblastic leukemia (ALL) where asparagine levels affect cancer cell survival. Targeting ASNS can influence chemotherapeutic strategies given its role in providing asparagine to leukemic cells. Additionally ASNS deficiency can lead to severe neurological conditions further highlighting its importance in human health. Its interaction with glutaminase is particularly relevant as both are involved in pathways targeted in ALL treatments.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information ASNS

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed36291140

2022

Targeting Asparagine Synthetase in Tumorgenicity Using Patient-Derived Tumor-Initiating Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gen Nishikawa,Kenji Kawada,Keita Hanada,Hisatsugu Maekawa,Yoshiro Itatani,Hiroyuki Miyoshi,Makoto Mark Taketo,Kazutaka Obama

Cancer research 80:5478-5490 PubMed33106333

2020

Brain Tumor Stem Cell Dependence on Glutaminase Reveals a Metabolic Vulnerability through the Amino Acid Deprivation Response Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ian J Restall,Orsolya Cseh,Laura M Richards,Trevor J Pugh,H Artee Luchman,Samuel Weiss

Cellular and molecular gastroenterology and hepatology 9:1-13 PubMed31421261

2019

Asparagine Synthetase Is Highly Expressed at Baseline in the Pancreas Through Heightened PERK Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Amitava Mukherjee,Nayyar Ahmed,Fateema T Rose,Abraheem N Ahmad,Tanveer A Javed,Li Wen,Rita Bottino,Xiangwei Xiao,Michael S Kilberg,Sohail Z Husain

The American journal of pathology 183:745-57 PubMed23886890

2013

Molecular and functional alterations in a mouse cardiac model of Friedreich ataxia: activation of the integrated stress response, eIF2α phosphorylation, and the induction of downstream targets.

Applications

Species

Mouse

Michael Li-Hsuan Huang,Sutharshani Sivagurunathan,Samantha Ting,Patric J Jansson,Christopher J D Austin,Matthew Kelly,Christopher Semsarian,Daohai Zhang,Des R Richardson

PloS one 5:e13892 PubMed21085483

2010

Cell-cycle inhibition by Helicobacter pylori L-asparaginase.

Applications

WB, IHC-P

Species

Human, Human

Claudia Scotti,Patrizia Sommi,Maria Valentina Pasquetto,Donata Cappelletti,Simona Stivala,Paola Mignosi,Monica Savio,Laurent Roberto Chiarelli,Giovanna Valentini,Victor M Bolanos-Garcia,Douglas Scott Merrell,Silvia Franchini,Maria Luisa Verona,Cristina Bolis,Enrico Solcia,Rachele Manca,Diego Franciotta,Andrea Casasco,Paola Filipazzi,Elisabetta Zardini,Vanio Vannini

Cancer research 68:1997-2005 PubMed18339882

2008

Histamine suppresses fibulin-5 and insulin-like growth factor-II receptor expression in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zoltan Pos,Zoltan Wiener,Peter Pocza,Melinda Racz,Sara Toth,Zsuzsanna Darvas,Viktor Molnar,Hargita Hegyesi,Andras Falus

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Asparagine synthetase 抗体 [EP282Y]

Anti-Asparagine synthetase antibody [EP282Y]

Western blot - Anti-Asparagine synthetase antibody [EP282Y] (AB40850)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (6)

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Abcam product promise