Anti-ASK1 (phospho S83) 抗体 (ab47304)
Key features and details
- Rabbit polyclonal to ASK1 (phospho S83)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ASK1 (phospho S83) antibody
ASK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ASK1 (phospho S83) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human ASK1 aa 1-100 (phospho S83).
Database link: Q99683 -
ポジティブ･コントロール
- Human breast carcinoma tissue and TNF alpha stimulated K562 cell extracts
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47304の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa).
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| IHC-P | (1) |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death. -
組織特異性
Abundantly expressed in heart and pancreas. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Dephosphorylated and activated by PGAM5. - Information by UniProt
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参照データベース
- Entrez Gene: 4217 Human
- Omim: 602448 Human
- SwissProt: Q99683 Human
- Unigene: 186486 Human
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別名
- Apoptosis signal regulating kinase 1 antibody
- Apoptosis signal-regulating kinase 1 antibody
- ASK 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASK1 (phospho S83) antibody (ab47304)ab47304 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.
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Western blot - Anti-ASK1 (phospho S83) antibody (ab47304)All lanes : Anti-ASK1 (phospho S83) antibody (ab47304)
Lane 1 : TNF alpha stimulated K562 cells
Lane 2 : K562 cells
Predicted band size: 155 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab47304 は 5 報の論文で使用されています。
- Kahraman DC et al. A new triazolothiadiazine derivative inhibits stemness and induces cell death in HCC by oxidative stress dependent JNK pathway activation. Sci Rep 12:15139 (2022). PubMed: 36071119
- Wu P et al. Licochalcone a Induces ROS-Mediated Apoptosis through TrxR1 Inactivation in Colorectal Cancer Cells. Biomed Res Int 2020:5875074 (2020). PubMed: 32596335
- Wu T et al. The IRE1 signaling pathway is involved in the protective effect of low-dose LPS on myocardial ischemia-reperfusion injury. Life Sci 231:116569 (2019). PubMed: 31202841
- Aytaç PS et al. Novel triazolothiadiazines act as potent anticancer agents in liver cancer cells through Akt and ASK-1 proteins. Bioorg Med Chem 24:858-72 (2016). PubMed: 26810835
- Liangpunsakul S et al. Imipramine blocks ethanol-induced ASMase activation, ceramide generation, and PP2A activation, and ameliorates hepatic steatosis in ethanol-fed mice. Am J Physiol Gastrointest Liver Physiol 302:G515-23 (2012). PubMed: 22194417