Anti-ASIC1 抗体 [EPR25411-161] (ab300563)

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Western blot - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR25411-161] to ASIC1
Suitable for: WB, IP, IHC-P
Reacts with: Mouse, Rat

Carrier Free

製品名

Anti-ASIC1 antibody [EPR25411-161]
ASIC1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR25411-161] to ASIC1
由来種

Rabbit
アプリケーション

適用あり: WB, IP, IHC-Pmore details
適用なし: Flow Cyt (Intra) or ICC/IF
種交差性

交差種: Mouse, Rat
非交差種: Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Tissue lysates: Mouse cerebellum, brain and spinal cord; Rat brain and spinal cord. IHC-P: Mouse and rat dorsal root ganglion, HEK-293T transfected with a human ASIC1 expression vector. IP: Mouse cerebellum tissue lysate
特記事項
ab300563 does not react in ICC and intracellular flow cytometry with mouse and rat species.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR25411-161
アイソタイプ

IgG
研究分野
- Neuroscience
- Processes

Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab300563の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		1/1000. Predicted molecular weight: 59 kDa.
IP		1/30.
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

特記事項
WB 1/1000. Predicted molecular weight: 59 kDa.
IP 1/30.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

追加情報

Is unsuitable for Flow Cyt (Intra) or ICC/IF.

機能

Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.
組織特異性

Expressed in most or all neurons.
配列類似性

Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN2 subfamily.
翻訳後修飾

Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel.
細胞内局在

Cell membrane. Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4.
Target information above from: UniProt accession P78348 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 11419 Mouse
- Entrez Gene: 79123 Rat
- SwissProt: Q6NXK8 Mouse
- SwissProt: P55926 Rat
- Unigene: 440107 Mouse
- Unigene: 37385 Rat
別名
- ACCN2 antibody
- ACCN2_HUMAN antibody
- Acid sensing ion channel 1 antibody
- Acid sensing ion channel 1a protein antibody
- Acid sensing ion channel antibody
- Acid-sensing ion channel 1 antibody
- AI843610 antibody
- Amiloride sensitive cation channel 2 neuronal antibody
- Amiloride-sensitive cation channel 2 antibody
- ASIC antibody
- ASIC1 antibody
- ASIC1A antibody
- B530003N02Rik antibody
- BNaC2 antibody
- Brain sodium channel 2 antibody
- Cation channel amiloride sensitive neuronal 2 antibody
- hBNaC2 antibody
- neuronal antibody
see all

Western blot - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

All lanes : Anti-ASIC1 antibody [EPR25411-161] (ab300563) at 1/1000 dilution

Lane 1 : Mouse cerebellum tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse spinal cord tissue lysate
Lane 4 : Mouse heart tissue lysate
Lane 5 : Mouse kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : Goat anti-Rabbit IgG (HRP) with minimal cross reactivity with human IgG at 1/2000 dilution
Lanes 2-5 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 59 kDa
Observed band size: 70, 150 kDa why is the actual band size different from the predicted?

Exposure time: 48 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID: 19218436, PMID: 26252376).

Negative controls: mouse heart, kidney (PMID: 9037075).
Western blot - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

All lanes : Anti-ASIC1 antibody [EPR25411-161] (ab300563) at 1/1000 dilution

Lane 1 : Rat brain tissue lysate
Lane 2 : Rat spinal cord tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 59 kDa
Observed band size: 70, 150 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID: 19218436, PMID: 26252376).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Immunohistochemical analysis of paraffin-embedded mouse dorsal root ganglion tissue labeling ASIC1 with ab300563 at 1/1000 dilution (0.498 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining in mouse dorsal root ganglion neurons. The section was incubated with ab300563 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue labeling ASIC1 with ab300563 at 1/1000 dilution (0.498 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining in rat dorsal root ganglion neurons. The section was incubated with ab300563 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Immunohistochemical analysis of paraffin-embedded HEK-293T cells labeling ASIC1 with ab300563 at 1/5000 dilution (0.1 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on (A) HEK-293T transfected with a human ASIC1 expression vector, no staining on (B) HEK-293T cells transfected with a human ASIC2a expression vector and (C) HEK-293T transfected with an empty vector. The section was incubated with ab300563 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling ASIC1 with ab300563 at 1/1000 dilution (0.498 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining in mouse cardiac muscle. The section was incubated with ab300563 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling ASIC1 with ab300563 at 1/1000 dilution (0.498 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining in rat cardiac muscle. The section was incubated with ab300563 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunoprecipitation - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

ASIC1 was immunoprecipitated from 0.35 mg mouse cerebellum tissue lysate with ab300563 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab300563 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse cerebellum tissue lysate 10 μg

Lane 2: ab300563 IP in mouse cerebellum tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300563 in mouse cerebellum tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes
Anti-ASIC1 antibody [EPR25411-161] (AB300563)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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ab300563 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300563 は論文での使用が確認できていません。

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Compatible Secondaries

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (0)

レビューと Q&A