Anti-ASCC2 抗体 (ab168811)
Key features and details
- Rabbit polyclonal to ASCC2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ASCC2 antibody
ASCC2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ASCC2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Snake, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
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免疫原
Synthetic peptide, corresponding to a region within amino acids 707-757 of Human ASCC2 (NP_115580.2).
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ポジティブ･コントロール
- HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab168811の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 86 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 86 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Enhances NF-kappa-B, SRF and AP1 transactivation. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the ASCC2 family.
Contains 1 CUE domain. - Information by UniProt
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参照データベース
- Entrez Gene: 458747 Chimpanzee
- Entrez Gene: 505503 Cow
- Entrez Gene: 486348 Dog
- Entrez Gene: 101133304 Gorilla
- Entrez Gene: 100058860 Horse
- Entrez Gene: 84164 Human
- Entrez Gene: 75452 Mouse
- Entrez Gene: 100444460 Orangutan
see all -
別名
- 1700011I11Rik antibody
- 2610034L15Rik antibody
- Activating signal cointegrator 1 complex subunit 2 antibody
see all
画像
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Western blot - Anti-ASCC2 antibody (ab168811)All lanes : Anti-ASCC2 antibody (ab168811) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-ASCC2 antibody (ab168811)Detection of ASCC2 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168811 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab168811 は 2 報の論文で使用されています。
- Wan L et al. Translation stress and collided ribosomes are co-activators of cGAS. Mol Cell 81:2808-2822.e10 (2021). PubMed: 34111399
- Chen H et al. Sinomenine Attenuates Acetaminophen-Induced Acute Liver Injury by Decreasing Oxidative Stress and Inflammatory Response via Regulating TGF-ß/Smad Pathway in vitro and in vivo. Drug Des Devel Ther 14:2393-2403 (2020). PubMed: 32606606