Anti-ASC2/ASCI 抗体 (ab47092)
Key features and details
- Rabbit polyclonal to ASC2/ASCI
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ASC2/ASCI antibody -
製品の詳細
Rabbit polyclonal to ASC2/ASCI -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Human ASC2/ASCI.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47092の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/2000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
1/1000 - 1/2000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Associates with apoptosis-associated specklike protein containing a CARD domain (ASC) and modulates its ability to collaborate with pyrin and cryopyrin in NF-kappa-B and pro-caspase-1 activation. Suppresses kinase activity of NF-kappa-B inhibitor kinase (IKK) complex, expression of NF-kappa-B inducible genes and inhibits NF-kappa-B activation by cytokines and LPS. -
組織特異性
Predominantly expressed in monocytes, macrophages and granulocytes. -
配列類似性
Contains 1 DAPIN domain. -
翻訳後修飾
Phosphorylated. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 260434 Human
- SwissProt: Q8WXC3 Human
- Unigene: 58314 Human
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別名
- PAAD domain only protein antibody
- PAAD-only protein 1 antibody
- POP1 antibody
see all
画像
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Western blot - Anti-ASC2/ASCI antibody (ab47092)All lanes : Anti-ASC2/ASCI antibody (ab47092) at 1/2000 dilution
Lane 1 : HEK-293 cells transiently transfected with plasmid encoding ASC2/ASCI
Lane 2 : HEK-293 cells transiently transfected with plasmid encoding POP1
Lane 3 : HEK-293 cells transiently transfected with a negative control plasmid
Predicted band size: 10 kDa
Observed band size: 10 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASC2/ASCI antibody (ab47092)Immunhistochemical analysis of ASC2/ASCI/POP1 expression in mouse spleen using ab47092 at 1:2000. Left and Right images: spleen at low and high magnification, respectively. ASC2/ASCI/POP1 staining is seen in the granulocytes, whereas the lymph and megakaryocytes are negative.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASC2/ASCI antibody (ab47092)Immunhistochemical analysis of ASC2/ASCI/POP1 expression in mouse using ab47092 at 1:2000. A, prostate tissue from a male mouse prostatitis. B, kidney from a mouse with pyelonephritis. A1 and B1, high magnification of A and B, respectively. ASC2/ASCI/POP1 staining is seen in the granulocytes
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (17)
ab47092 は 17 報の論文で使用されています。
- Liu F et al. Nigakinone alleviates DSS-induced experimental colitis via regulating bile acid profile and FXR/NLRP3 signaling pathways. Phytother Res 37:15-34 (2023). PubMed: 36054406
- He J et al. Astragaloside IV Attenuates High-Glucose-Induced Impairment in Diabetic Nephropathy by Increasing Klotho Expression via the NF-κB/NLRP3 Axis. J Diabetes Res 2023:7423661 (2023). PubMed: 37261217
- Dong D et al. VX-765 Alleviates β-Amyloid Deposition and Secondary Degeneration in the Ipsilateral Hippocampus and Ameliorates Cognitive Decline after Focal Cortical Infarction in Rats. J Mol Neurosci 72:2389-2397 (2022). PubMed: 36441377
- Shen J et al. Triptolide improves myocardial fibrosis in rats through inhibition of nuclear factor kappa B and NLR family pyrin domain containing 3 inflammasome pathway. Korean J Physiol Pharmacol 25:533-543 (2021). PubMed: 34697264
- Zhou W et al. MicroRNA-1929-3p participates in murine cytomegalovirus-induced hypertensive vascular remodeling through Ednra/NLRP3 inflammasome activation. Int J Mol Med 47:719-731 (2021). PubMed: 33416142
