Anti-ASC1 抗体 (ab70627)
Key features and details
- Rabbit polyclonal to ASC1
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ASC1 antibody
ASC1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ASC1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla
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免疫原
Synthetic peptide, corresponding to amino acids 225-275 of Human ASC1 (NP_057297.2)
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ポジティブ･コントロール
- HeLa whole cell lysate (ab150035) 293T whole cell lysate NIH3T3 whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70627の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (2) |
1/1000 - 1/5000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000 - 1/5000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Transcription coactivator of nuclear receptors which functions in conjunction with CBP-p300 and SRC-1 and may play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. Plays a role in androgen receptor transactivation and in testicular function. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasmic under conditions of serum deprivation. - Information by UniProt
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参照データベース
- Entrez Gene: 9325 Human
- Entrez Gene: 56404 Mouse
- Omim: 604501 Human
- SwissProt: Q15650 Human
- SwissProt: Q9QXN3 Mouse
- Unigene: 500340 Human
- Unigene: 208379 Mouse
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別名
- Activating signal cointegrator 1 antibody
- ASC 1 antibody
- ASC-1 antibody
see all
画像
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Immunoprecipitation - Anti-ASC1 antibody (ab70627)
ASC1 was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab70627at 3 µg per reaction. Western blot was performed on the immunoprecipitate using ab70627 at 1 µg/mL.
Lane 1: ab70627 IP in HeLa whole cell lysate.
Lane 2: ab70627 IP in HeLa whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes
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Western blot - Anti-ASC1 antibody (ab70627)All lanes : Anti-ASC1 antibody (ab70627) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 66 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASC1 antibody (ab70627)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling ASC1 with ab70627 at 1/1000 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab70627 は 11 報の論文で使用されています。
- Zhao N et al. MALAT1 knockdown alleviates the pyroptosis of microglias in diabetic cerebral ischemia via regulating STAT1 mediated NLRP3 transcription. Mol Med 29:44 (2023). PubMed: 37013491
- Ouyang R et al. FGF21 attenuates high uric acid‑induced endoplasmic reticulum stress, inflammation and vascular endothelial cell dysfunction by activating Sirt1. Mol Med Rep 25:N/A (2022). PubMed: 34850960
- Liu L et al. Epac1 and PKA agonists inhibit ROS to reduce NLRP3 inflammasome proteins in retinal endothelial cells. Mol Vis 28:500-506 (2022). PubMed: 37089701
- Sun J et al. LncRNA H19 activates cell pyroptosis via the miR-22-3p/NLRP3 axis in pneumonia. Am J Transl Res 13:11384-11398 (2021). PubMed: 34786066
- Yang M et al. Colchicine Alleviates Cholesterol Crystal-Induced Endothelial Cell Pyroptosis through Activating AMPK/SIRT1 Pathway. Oxid Med Cell Longev 2020:9173530 (2020). PubMed: 32733639