Anti-ASAH2 抗体 (ab63804)
Key features and details
- Rabbit polyclonal to ASAH2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ASAH2 antibody
ASAH2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ASAH2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide surrounding amino acid 741 of human ASAH2
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ポジティブ･コントロール
- Jurkat cell lysate.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 3828 ASAH2 Antibody. 3828-100 is the same size as the 100 µg size of ab63804.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63804の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa). |
ターゲット情報
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機能
Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. -
組織特異性
Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B. -
配列類似性
Belongs to the neutral ceramidase family. -
翻訳後修飾
N-glycosylated. Required for enzyme activity.
O-glycosylated. Required to retain it as a type II membrane protein at the cell surface.
Phosphorylated. May prevent ubiquitination and subsequent degradation.
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxid. -
細胞内局在
Cell membrane. The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N-terminus, which may explain this localization which probably does not exist in vivo. - Information by UniProt
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参照データベース
- Entrez Gene: 56624 Human
- Entrez Gene: 653308 Human
- Omim: 611202 Human
- SwissProt: Q9NR71 Human
- Unigene: 512645 Human
- Unigene: 710005 Human
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別名
- Acylsphingosine deacylase 2 antibody
- AI585898 antibody
- ASAH2 antibody
see all
画像
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Western blot - Anti-ASAH2 antibody (ab63804)All lanes : Anti-ASAH2 antibody (ab63804) at 4 µg/ml
All lanes : Jurkat cell lysate
Predicted band size: 86 kDa
Observed band size: 86 kDa
Additional bands at: 68 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab63804 は 1 報の論文で使用されています。
- Zhu Q et al. Low-dose cytokine-induced neutral ceramidase secretion from INS-1 cells via exosomes and its anti-apoptotic effect. FEBS J 281:2861-70 (2014). PubMed: 24798654