Anti-ASAH1 抗体 [23/Acid Ceramidase] - BSA and Azide free (ab302634)
![Western blot - Anti-ASAH1 antibody [23/Acid Ceramidase] - BSA and Azide free (AB302634)](https://www.abcam.co.jp/ps/products/302/ab302634/Images/AB302634-490746-anti-asah1-antibody-23-acid-ceramidase-western-blot-human-skin-sk-mel-28-a375.jpg "Western blot - Anti-ASAH1 antibody [23/Acid Ceramidase] - BSA and Azide free (AB302634)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [23/Acid Ceramidase] to ASAH1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ASAH1 antibody [23/Acid Ceramidase] - BSA and Azide free
ASAH1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [23/Acid Ceramidase] to ASAH1 - BSA and Azide free -
由来種
Mouse -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Human skin tissue lysate; SK-MEL-28, A375 whole cell lysates.
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特記事項
ab302634 is a carrier free version of ab302633.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.20
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
23/Acid Ceramidase -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302634の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 55, 13 kDa (predicted molecular weight: 45 kDa).
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| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 55, 13 kDa (predicted molecular weight: 45 kDa). |
ターゲット情報
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機能
Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. -
組織特異性
Broadly expressed with highest expression in heart. -
関連疾患
Defects in ASAH1 are the cause of Farber lipogranulomatosis (FL) [MIM:228000]; also known as Farber disease (FD). This sphingolipid disease is characterized by subcutaneous lipid-loaded nodules, excruciating pain in the joints and extremities, marked accumulation of ceramide in lysosomes, and death by three years of age. -
配列類似性
Belongs to the acid ceramidase family. -
細胞内局在
Lysosome. - Information by UniProt
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参照データベース
- Entrez Gene: 427 Human
- Omim: 613468 Human
- SwissProt: Q13510 Human
- Unigene: 527412 Human
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別名
- AC antibody
- ACDase antibody
- Acid CDase antibody
see all
画像
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Western blot - Anti-ASAH1 antibody [23/Acid Ceramidase] - BSA and Azide free (AB302634)All lanes : Anti-ASAH1 antibody [23/Acid Ceramidase] (ab302633) at 1/1000 dilution
Lane 1 : Human skin tissue lysate
Lane 2 : SK-MEL-28 (human malignant melanoma), whole cell lysate
Lane 3 : A375 (human malignant melanoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 13,55 kDa why is the actual band size different from the predicted?This data was developed using ab302633, the same antibody clone in a different buffer formulation.
Blocking / Diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
Lane 1: 3 minutes
Lane 2, 3: 70 secondsNegative control: A375 (PMID: 26553872, 30254208);
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID:7744740, 11451951). -
![Anti-ASAH1 antibody [23/Acid Ceramidase] - BSA and Azide free (AB302634)](https://www.abcam.co.jp/ps/products/302/ab302634/Images/AB302634-490744-Benefits-of-Recombinant-antibodies.jpg)
Anti-ASAH1 antibody [23/Acid Ceramidase] - BSA and Azide free (AB302634)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab302634 は論文での使用が確認できていません。
