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AB77586

Anti-ARSA/ASA 抗体

Anti-ARSA/ASA antibody

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(1 Publication)

Goat Polyclonal ARSA/ASA antibody. Suitable for IHC-P, ICC, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARSA aa 400-450.

別名を表示する

Arylsulfatase A, ASA, Cerebroside-sulfatase, ARSA

3 Images
Flow Cytometry (Intracellular) - Anti-ARSA/ASA antibody (AB77586)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ARSA/ASA antibody (AB77586)

Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling ARSA/ASA with ab77586 at 10 μg/mL. Cells were permeabilised with 0.5% Triton. Alexa Fluor 488 secondary antibody (1ug/ml). Unimmunized goat IgG was used as the isotype control (black).

Immunocytochemistry - Anti-ARSA/ASA antibody (AB77586)
  • ICC

Supplier Data

Immunocytochemistry - Anti-ARSA/ASA antibody (AB77586)

Immunofluorescence analysis of HeLa cells labelling ARSA/ASA with ab77586 at 10 µg/mL. Cells were permeabilized with 0.15% Triton X-100. Alexa Fluor 488 secondary antibody (2ug/ml). Nuclear DNA was labelled with DAPI (blue). Negative control : Unimmunized goat IgG 10 µg/mL.

Western blot - Anti-ARSA/ASA antibody (AB77586)
  • WB

Supplier Data

Western blot - Anti-ARSA/ASA antibody (AB77586)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-ARSA/ASA antibody (ab77586) at 0.5 µg/mL

All lanes:

Mouse Testis lysate (in RIPA buffer) at 35 µg

Predicted band size: 54 kDa

Observed band size: 54 kDa

false

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

WB, Flow Cyt (Intra), ICC, IHC-P

applications

免疫原

Synthetic Peptide within Human ARSA aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

P15289

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p><p>Approx 60-65Da band observed in Mouse and Rat Testis lysates.</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
ab77586 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
バッファー組成
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The ARSA protein also known as arylsulfatase A or ASA protein functions mechanically as a lysosomal enzyme responsible for the cleavage of cerebroside sulfate into cerebroside and sulfate. It belongs to a family of enzymes important for the hydrolysis of sulfate esters and has a molecular mass of about 62 kDa. This enzyme is expressed mainly in the liver and kidney but also in the brain where it plays important roles in normal functioning.
Biological function summary

ARSA is essential in the breakdown of sulfatides which are components of myelin sheaths in the central nervous system. It functions as part of a lysosomal complex involving cooperators like saposin B which facilitates its interaction with sulfatide substrates. By breaking down sulfatides ARSA helps in cellular membrane maintenance and recycling contributing to neurological function and stability.

Pathways

ARSA is mainly involved in the sphingolipid metabolism pathway. This pathway manages the degradation of sphingolipids important for maintaining neuron and cell membrane integrity. ARSA also interacts with proteins such as galactosylceramidase as both play roles in lipid recycling and are essential for maintaining cell health within the nervous system.

ARSA's deficiency is a well-known factor in metachromatic leukodystrophy (MLD) a genetic disorder leading to progressive leukodystrophy. This condition results from sulfatide accumulation in the nervous system caused by insufficient ARSA activity. The disease involves other proteins such as saposin B which when dysfunctional exacerbate ARSA-related pathologies and symptoms. Another disorder related to defective ARSA function includes chronic kidney disease where compromised sulfatide metabolism further effects cellular processes.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Hydrolyzes cerebroside sulfate.
See full target information ARSA
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 25:3182-3193 PubMed33611830

2021

MARCH5 restores endothelial cell function against ischaemic/hypoxia injury via Akt/eNOS pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhua Lei,Junli Li,Changming Li,Li Chen,Fangyang Huang,Dan Xiao,Jialiang Zhang,Jiahao Zhao,Guoyong Li,Tianyi Qu,Hao Zhou,Yanbiao Liao,Mao Chen
View all publications
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代替製品

Primary Antibodies

AB74804

Anti-ARSA/ASA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARSA/ASA antibody (AB74804)

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Primary Antibodies

AB67089

Anti-ARSA/ASA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARSA/ASA antibody (AB67089)

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Primary Antibodies

AB154300

Anti-ARSA/ASA antibody

Western blot - Anti-ARSA/ASA antibody (AB154300)

  • 0
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Primary Antibodies

AB174844

Anti-ARSA/ASA antibody [EPR11039]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARSA/ASA antibody [EPR11039] (AB174844)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB240638

Anti-GALC antibody [EPR23598-126]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALC antibody [EPR23598-126] (AB240638)

  • 0
  • 0
Primary Antibodies

AB169480

Anti-FUCA2 antibody

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  • 0
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Assay Kits

AB253371

Lysosomal Galactocerebrosidase (GALC) Analysis Kit

Fluorescent Cell Imaging - Lysosomal Galactocerebrosidase (GALC) Analysis Kit (AB253371)

  • 0
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Secondary Antibodies

AB150129

Donkey Anti-Goat IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (AB150129)

  • 5
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