Anti-ARPC2 抗体 (ab11798)
Key features and details
- Goat polyclonal to ARPC2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ARPC2 antibody
ARPC2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ARPC2 -
由来種
Goat -
特異性
This antibody is expected to recognise both reported isoforms as represented by NP_005722 and NP_690601. -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Zebrafish
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免疫原
Synthetic peptide: EMKTITGKTFSSR, corresponding to amino acids 288-300 of Human ARPC2.
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ポジティブ･コントロール
- WB: Caco-2, HeLa and Jurkat cell lysates. IHC-P: Human Muscle lysates. ICC/IF: HeLa and A431 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11798の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 34 kDa.
A 1 hour primary incubation at room temperature is recommended for this product. |
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| ICC/IF |
Use a concentration of 10 µg/ml.
PubMed ID: 22021442 |
| 特記事項 |
|---|
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IHC-P
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 34 kDa. A 1 hour primary incubation at room temperature is recommended for this product. |
|
ICC/IF
Use a concentration of 10 µg/ml. PubMed ID: 22021442 |
ターゲット情報
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機能
Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament. -
配列類似性
Belongs to the ARPC2 family. -
細胞内局在
Cytoplasm > cytoskeleton. Cell projection. - Information by UniProt
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参照データベース
- Entrez Gene: 10109 Human
- Entrez Gene: 301511 Rat
- Entrez Gene: 327068 Zebrafish
- Omim: 604224 Human
- SwissProt: O15144 Human
- SwissProt: P85970 Rat
- Unigene: 529303 Human
- Unigene: 93317 Rat
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別名
- Actin related protein 2/3 complex subunit 2 34kDa antibody
- Actin related protein 2/3 complex subunit 2 antibody
- Actin-related protein 2/3 complex subunit 2 antibody
see all
画像
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Western blot - Anti-ARPC2 antibody (ab11798)Lane 1 : Anti-ARPC2 antibody (ab11798) at 0.5 µg/ml
Lanes 2-3 : Anti-ARPC2 antibody (ab11798) at 0.1 µg/ml
Lane 1 : CACO-2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 35 µg per lane.
Observed band size: 34-35 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody (ab11798)ab11798 (3.8 µg/ml) staining ARPC2 in paraffin-embedded Human spleen tissue following steamed antigen retrieval with citrate buffer (pH6). -
Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)Immunocytochemistry/ Immunofluorescence analysis of A431 cells labeling ARPC2 with ab11798 at 10 µg/mL (1 hour) showing cytoplasmic statining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 at 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
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Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling ARPC2 with ab11798 at 10 µg/mL (1 hour) showing cytoplasmic statining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 at 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (5)
ab11798 は 5 報の論文で使用されています。
- Xing H et al. Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction. Sci Rep 12:22474 (2022). PubMed: 36577792
- Cheng Z et al. ARPC2 promotes breast cancer proliferation and metastasis. Oncol Rep 41:3189-3200 (2019). PubMed: 31002363
- Xia P et al. WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner. J Exp Med 211:2119-34 (2014). PubMed: 25225459
- Chauhan BK et al. Balanced Rac1 and RhoA activities regulate cell shape and drive invagination morphogenesis in epithelia. Proc Natl Acad Sci U S A 108:18289-94 (2011). ICC/IF ; Mouse . PubMed: 22021442
- Li Y et al. The profilin:actin complex localizes to sites of dynamic actin polymerization at the leading edge of migrating cells and pathogen-induced actin tails. Eur J Cell Biol 87:893-904 (2008). PubMed: 18707793
