Anti-ARPC1B 抗体 (ab115217)
Key features and details
- Goat polyclonal to ARPC1B
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ARPC1B antibody
ARPC1B 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ARPC1B -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Zebrafish
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免疫原
Synthetic peptide corresponding to Human ARPC1B aa 1-100 (N terminal).
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ポジティブ･コントロール
- Human Thymus tissue, Human liver lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115217の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 41 kDa.
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| IHC-P |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 41 kDa. |
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IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. -
配列類似性
Belongs to the WD repeat ARPC1 family.
Contains 6 WD repeats. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 416490 Chicken
- Entrez Gene: 326600 Cow
- Entrez Gene: 10095 Human
- Entrez Gene: 11867 Mouse
- Entrez Gene: 100174964 Pig
- Entrez Gene: 54227 Rat
- Omim: 604223 Human
- SwissProt: Q58CQ2 Cow
see all -
別名
- Actin related protein 2/3 complex subunit 1B 41kDa antibody
- Actin related protein 2/3 complex subunit 1B antibody
- Actin-related protein 2/3 complex subunit 1B antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC1B antibody (ab115217)Human thymus (formalin fixed, paraffin embedded) stained with ab115217 at 2.5 ug/ml followed by biotinylated anti Goat IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.
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Western blot - Anti-ARPC1B antibody (ab115217)Anti-ARPC1B antibody (ab115217) at 2 µg/ml + Human Liver lysate in RIPA buffer at 35 µg
Predicted band size: 41 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab115217 は 1 報の論文で使用されています。
- Chen Z et al. Single-cell sequencing reveals homogeneity and heterogeneity of the cytopathological mechanisms in different etiology-induced AKI. Cell Death Dis 14:318 (2023). PubMed: 37169762