AB119766

Anti-Arp2 (phospho T237 + T238) 抗体

Name: Anti-Arp2 (phospho T237 + T238) 抗体 (ab119766) | Abcam
Brand: Abcam Limited
SKU: AB119766
Price: 76000 JPY
Availability: InStock

Anti-Arp2 (phospho T237 + T238) antibody

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(3 Publications)

Rabbit Polyclonal ARP2 phospho T237 + T238 antibody. Suitable for ICC, WB and reacts with Rat, Human samples. Cited in 3 publications.

別名を表示する

ARP2, ACTR2, Actin-related protein 2, Actin-like protein 2

2 Images

Unknown

Immunocytochemistry - Anti-Arp2 (phospho T237 + T238) antibody (AB119766)

ab119766 at 1/100 dilution staining Arp2 (phospho T237 + T238) in rat PC12 cells differentiated with NGF (right).
Detection used an appropriate secondary antibody conjugated to Cy3.

Unknown

Western blot - Anti-Arp2 (phospho T237 + T238) antibody (AB119766)

All lanes:

Western blot - Anti-Arp2 (phospho T237 + T238) antibody (ab119766) at 1/1000 dilution

Lane 1:

A431 cells treated with Calyculin A(100 nM) for 30 min

Lane 2:

A431 cells treated with Calyculin A(100 nM) for 30 min and treated with lambda phosphatase

Predicted band size: 45 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Human

アプリケーション

ICC, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Fish": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

Do not aliquot.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Arp2 also known as actin-related protein 2 is a subunit of the Arp2/3 complex which plays an important role in nucleating actin filaments. This protein facilitates the formation of branched actin networks important for cellular functions. Arp2 has a mass of approximately 44 kDa and is widely expressed across different tissues especially in regions with significant actin cytoskeletal remodeling such as the cortex of cells.

Biological function summary

Arp2 acts as a central component within the Arp2/3 complex which regulates actin polymerization and branching. This complex consists of seven subunits and Arp2 in concert with Arp3 and additional regulatory proteins initiates new actin filament branches from pre-existing ones. As a result it is essential for cellular processes like cell movement phagocytosis and maintaining cell shape by controlling the spatial and temporal organization of actin filaments.

Pathways

The Arp2/3 complex is integral to the actin polymerization pathway and the pathway for cell motility. Arp2 influences the dynamics of filamentous actin interacting with proteins such as Wiskott-Aldrich syndrome protein (WASP) to promote branching. This process affects various cellular activities linked to actin forming connections with the Rho GTPase signaling network which regulates cytoskeletal dynamics and cellular signaling pathways.

Disruptions in the function of the Arp2/3 complex have been associated with Wiskott-Aldrich syndrome and cancer metastasis. Wiskott-Aldrich syndrome linked with mutations in the WASP protein shows impaired actin branching capability due to insufficient Arp2/3 complex activity. Additionally aberrant regulation of actin polymerization in cancer cells leads to enhanced invasive properties implicating Arp2 as a potential factor in the pathology of metastatic progression.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9000076). Seems to contact the pointed end of the daughter actin filament (PubMed : 9000076). In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation (PubMed : 29058690). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 17220302, PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).

See full target information ACTR2 phospho T237 + T238

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 133: PubMed32107292

2020

Direct interaction between CEP85 and STIL mediates PLK4-driven directed cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Liu,Jaeyoun Kim,Reuben Philip,Vaishali Sridhar,Megha Chandrashekhar,Jason Moffat,Mark van Breugel,Laurence Pelletier

PLoS pathogens 14:e1007005 PubMed29668757

2018

Actin polymerization in the endosomal pathway, but not on the Coxiella-containing vacuole, is essential for pathogen growth.

Applications

Unspecified application

Species

Unspecified reactive species

Heather E Miller,Charles L Larson,Robert A Heinzen

Journal of immunology (Baltimore, Md. : 1950) 199:3176-3186 PubMed28947541

2017

Aging-Impaired Filamentous Actin Polymerization Signaling Reduces Alveolar Macrophage Phagocytosis of Bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Zhigang Li,Yang Jiao,Erica K Fan,Melanie J Scott,Yuehua Li,Song Li,Timothy R Billiar,Mark A Wilson,Xueyin Shi,Jie Fan

View all publications

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Abcam product promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-Arp2 (phospho T237 + T238) 抗体

Anti-Arp2 (phospho T237 + T238) antibody

Immunocytochemistry - Anti-Arp2 (phospho T237 + T238) antibody (AB119766)

Western blot - Anti-Arp2 (phospho T237 + T238) antibody (AB119766)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise