Anti-Arp2 抗体 [FMS96] (ab49674)
![Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody [FMS96] (ab49674)](https://www.abcam.co.jp/ps/products/49/ab49674/Images/ab49674-61765-anti-arp2-antibody-fms96-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody [FMS96] (ab49674)")
Key features and details
- Mouse monoclonal [FMS96] to Arp2
- Suitable for: WB, ICC/IF, Indirect ELISA
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-Arp2 antibody [FMS96]
Arp2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [FMS96] to Arp2 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, Indirect ELISAmore details -
種交差性
交差種: Mouse, Rat, Dog, Human -
免疫原
Recombinant full length protein corresponding to Human Arp2.
Database link: P61160 -
ポジティブ･コントロール
- WB: C2C12 whole cell lysate.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
FMS96 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab49674の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
|
| Indirect ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
Indirect ELISA
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. -
配列類似性
Belongs to the actin family. ARP2 subfamily. -
細胞内局在
Cytoplasm > cytoskeleton. Cell projection. - Information by UniProt
-
参照データベース
- Entrez Gene: 10097 Human
- Entrez Gene: 66713 Mouse
- Entrez Gene: 289820 Rat
- Omim: 604221 Human
- SwissProt: P61160 Human
- SwissProt: P61161 Mouse
- SwissProt: Q5M7U6 Rat
- Unigene: 643727 Human
see all -
別名
- Actin like protein 2 antibody
- Actin related protein 2 antibody
- Actin-like protein 2 antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody [FMS96] (ab49674)C2C12 cells were incubated with ab49674 at 10 µg/ml followed by Goat Anti Mouse IgG Alexa-488 (green staining). The red staining is obtained by staining actin with Alexa-546 phalloidin.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody [FMS96] (ab49674)](https://www.abcam.co.jp/ps/products/49/ab49674/Images/ab49674-61764-anti-arp2-antibody-fms96-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody [FMS96] (ab49674)Image from Aggarwal A et al., PLoS Pathog. 2012 Jun;8(6):e1002762. Epub 2012 Jun 7. Fig 4.; doi:10.1371/journal.ppat.1002762; June 7, 2012, PLoS Pathog 8(6): e1002762.Immunofluorescence analysis of dendritic cells infected with HIV, staining Arp2 (blue) with ab49674 at 5 mg/ml.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (15)
ab49674 は 15 報の論文で使用されています。
- Johnson JL et al. Differential dysregulation of granule subsets in WASH-deficient neutrophil leukocytes resulting in inflammation. Nat Commun 13:5529 (2022). PubMed: 36130971
- Saelens JW et al. An ancestral mycobacterial effector promotes dissemination of infection. Cell 185:4507-4525.e18 (2022). PubMed: 36356582
- González-Martín A et al. c-Abl regulates a synaptic plasticity-related transcriptional program involved in memory and learning. Prog Neurobiol 205:102122 (2021). PubMed: 34284000
- Kutsch M et al. Direct binding of polymeric GBP1 to LPS disrupts bacterial cell envelope functions. EMBO J 39:e104926 (2020). PubMed: 32510692
- Plessner M et al. Centrosomal Actin Assembly Is Required for Proper Mitotic Spindle Formation and Chromosome Congression. iScience 15:274-281 (2019). PubMed: 31096079