Anti-Arp2 抗体 [EPR7979] (ab129018)
![Western blot - Anti-Arp2 antibody [EPR7979] (ab129018)](https://www.abcam.co.jp/ps/products/129/ab129018/Images/ab129018-142940-anti-arp2-antibody-epr7979-western-blot.jpg "Western blot - Anti-Arp2 antibody [EPR7979] (ab129018)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7979] to Arp2
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Arp2 antibody [EPR7979]
Arp2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7979] to Arp2 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human Arp2 aa 1-100. The exact sequence is proprietary.
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ポジティブ･コントロール
- Jurkat, HUVEC, MCF-7, HeLa lysates. MCF-7 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数（KD 値）
KD = 1.75 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR7979 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab129018の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa).
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa). |
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.
ターゲット情報
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機能
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. -
配列類似性
Belongs to the actin family. ARP2 subfamily. -
細胞内局在
Cytoplasm > cytoskeleton. Cell projection. - Information by UniProt
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参照データベース
- Entrez Gene: 10097 Human
- Entrez Gene: 66713 Mouse
- Entrez Gene: 289820 Rat
- Omim: 604221 Human
- SwissProt: P61160 Human
- SwissProt: P61161 Mouse
- SwissProt: Q5M7U6 Rat
- Unigene: 643727 Human
see all -
別名
- Actin like protein 2 antibody
- Actin related protein 2 antibody
- Actin-like protein 2 antibody
see all
画像
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Western blot - Anti-Arp2 antibody [EPR7979] (ab129018)All lanes : Anti-Arp2 antibody [EPR7979] (ab129018) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 45 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-Arp2 antibody [EPR7979] (ab129018)](https://www.abcam.co.jp/ps/products/129/ab129018/Images/ab129018-142939-anti-arp2-antibody-epr7979-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Arp2 antibody [EPR7979] (ab129018)Intracellular flow cytometric analysis of permeabilized MCF-7 cells using ab129018 at 1/10 (red) or a rabbit IgG (negative) (green)
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![OI-RD Scanning - Anti-Arp2 antibody [EPR7979] (ab129018)](https://www.abcam.co.jp/ps/products/129/ab129018/Images/ab129018-142938-anti-arp2-antibody-epr7979.jpg)
OI-RD Scanning - Anti-Arp2 antibody [EPR7979] (ab129018) -
![Anti-Arp2 antibody [EPR7979] (ab129018)](https://www.abcam.co.jp/ps/products/129/ab129018/Images/ab129018-4-benefits-of-recombinant-antibodies.png)
Anti-Arp2 antibody [EPR7979] (ab129018)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab129018 は 4 報の論文で使用されています。
- Talukdar SN et al. RSV-induced expanded ciliated cells contribute to bronchial wall thickening. Virus Res 327:199060 (2023). PubMed: 36746339
- Davidson A et al. Salmonella invasion of a cell is self-limiting due to effector-driven activation of N-WASP. iScience 26:106643 (2023). PubMed: 37168569
- Kage F et al. Myosin II proteins are required for organization of calcium-induced actin networks upstream of mitochondrial division. Mol Biol Cell 33:ar63 (2022). PubMed: 35427150
- Mehedi M et al. Actin-Related Protein 2 (ARP2) and Virus-Induced Filopodia Facilitate Human Respiratory Syncytial Virus Spread. PLoS Pathog 12:e1006062 (2016). PubMed: 27926942