Anti-Arp2 抗体 (ab47654)
Key features and details
- Rabbit polyclonal to Arp2
- Suitable for: IP, WB, ICC/IF
- Reacts with: Mouse, Human, Xenopus laevis
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Arp2 antibody
Arp2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Arp2 -
由来種
Rabbit -
特異性
Replenishment batches of our polyclonal antibody, ab47654 are tested in WB. Previous batches were additionally validated in ICC/IF and IP. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab129018.
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アプリケーション
適用あり: IP, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human, Xenopus laevis
交差が予測される動物種: Rat, Chicken, Cow, Orangutan
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免疫原
Synthetic peptide corresponding to Human Arp2 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab47653) -
ポジティブ･コントロール
- This antibody gave a positive signal in the following whole cell lysates; HeLa (Human epithelial carcinoma cell line), Jurkat (Human T cell lymphoblast-like cell line), A431 (Human epithelial carcinoma cell line), HEK293 (Human embryonic kidney cell line), HepG2 (Human hepatocellular liver carcinoma cell line), MCF7 (Human breast adenocarcinoma cell line), and SHSY-5Y (Human neuroblastoma cell line).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47654の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | (1) |
Use at an assay dependent concentration.
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| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 特記事項 |
|---|
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IP
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
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ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. -
配列類似性
Belongs to the actin family. ARP2 subfamily. -
細胞内局在
Cytoplasm > cytoskeleton. Cell projection. - Information by UniProt
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参照データベース
- Entrez Gene: 396147 Chicken
- Entrez Gene: 538486 Cow
- Entrez Gene: 10097 Human
- Entrez Gene: 66713 Mouse
- Entrez Gene: 100173969 Orangutan
- Entrez Gene: 289820 Rat
- Entrez Gene: 443741 Xenopus laevis
- Omim: 604221 Human
see all -
別名
- Actin like protein 2 antibody
- Actin related protein 2 antibody
- Actin-like protein 2 antibody
see all
画像
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Western blot - Anti-Arp2 antibody (ab47654)All lanes : Anti-Arp2 antibody (ab47654) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Additional bands at: 27 kDa, 60 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody (ab47654)ICC/IF image of ab47654 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab47654, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HEK 293, HepG2 and MCF7 cells.
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Immunoprecipitation - Anti-Arp2 antibody (ab47654)Arp2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to Arp2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab47654.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 45kDa: Arp2.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (24)
ab47654 は 24 報の論文で使用されています。
- Sveeggen TM et al. Annexin A2 modulates phospholipid membrane composition upstream of Arp2 to control angiogenic sprout initiation. FASEB J 37:e22715 (2023). PubMed: 36527391
- Liu LZ et al. The activated CD36-Src axis promotes lung adenocarcinoma cell proliferation and actin remodeling-involved metastasis in high-fat environment. Cell Death Dis 14:548 (2023). PubMed: 37612265
- Bhanja A et al. N-WASP-dependent branched actin polymerization attenuates B-cell receptor signaling by increasing the molecular density of receptor clusters. Elife 12:N/A (2023). PubMed: 38085658
- Mitrentsi I et al. Heterochromatic repeat clustering imposes a physical barrier on homologous recombination to prevent chromosomal translocations. Mol Cell 82:2132-2147.e6 (2022). PubMed: 35447083
- Wang L et al. Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly. Cell Res 32:190-209 (2022). PubMed: 34782749