AB35604

Anti-Aromatase 抗体

Name: Anti-Aromatase 抗体 (ab35604) | Abcam
Brand: Abcam Limited
SKU: AB35604
Rating: 4 (5 reviews)

Anti-Aromatase antibody

(5 Reviews)

(16 Publications)

Rabbit Polyclonal Aromatase antibody. Suitable for WB and reacts with Human samples. Cited in 16 publications.

別名を表示する

ARO1, CYAR, CYP19, CYP19A1, Aromatase, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, Estrogen synthase

1 Images

Lab

Western blot - Anti-Aromatase antibody (AB35604)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab35604 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-Aromatase antibody (ab35604) at 1 µg/mL

All lanes:

Human placenta tissue lysate - total protein (ab29745) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 58 kDa

Observed band size: 55 kDa

true

Exposure time: 150s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Aromatase also known as CYP19A is an enzyme responsible for the conversion of androgens into estrogens. It is a member of the cytochrome P450 superfamily and has a molecular mass of approximately 58 kDa. Aromatase is mainly expressed in the gonads brain adipose tissue and placenta. The activity of this enzyme is important for estrogen biosynthesis which is especially important in postmenopausal women where adipose tissue becomes an important estrogen source.

Biological function summary

The enzyme catalyzes the aromatization of androgens aiding in the production of estrogens like estradiol. This process is essential for maintaining estrogen levels in the human body. Aromatase operates as a monomer and does not form part of any larger complex. The balance of androgens and estrogens maintained by aromatase activity plays a significant role in reproductive functions and other physiological processes.

Pathways

Aromatase enzyme activity plays a pivotal role in the steroidogenesis pathway which involves the biosynthesis of steroid hormones. It is also a part of the estrogen signaling pathway interacting with other proteins like estrogen receptors. These pathways help regulate gene expression and are important in processes such as cell proliferation and differentiation linking aromatase function to broader metabolic and developmental effects.

Aromatase has a significant role in conditions such as hormone receptor-positive breast cancer and endometriosis. In breast cancer increased aromatase activity elevates estrogen levels promoting cancer cell growth. Anti-aromatase drugs like aromatase inhibitors target this enzyme to lower estrogen production. Additionally defects in aromatase activity can lead to disorders like aromatase deficiency resulting in an imbalance in sex hormone levels. Understanding its function aids in the development of therapies targeting proteins like the estrogen receptor which interacts with products of aromatase activity.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively (PubMed : 27702664, PubMed : 2848247). Catalyzes three successive oxidations of C19 androgens : two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid (PubMed : 20385561). Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen (PubMed : 22773874). Also displays 2-hydroxylase activity toward estrone (PubMed : 22773874). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed : 20385561, PubMed : 22773874).

See full target information CYP19A1

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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Veterinary sciences 9: PubMed36006309

2022

Hormonal Homologies between Canine Mammary Cancer and Human Breast Cancer in a Series of Cases.

Applications

Unspecified application

Species

Unspecified reactive species

Paloma Jimena de Andrés,Sara Cáceres,Juan Carlos Illera,Belén Crespo,Gema Silván,Felisbina Luisa Queiroga,Maria José Illera,Maria Dolores Pérez-Alenza,Laura Peña

Frontiers in endocrinology 12:666195 PubMed34531822

2021

Extracellular Vesicles Inhibit Proliferation and Invasion of Ovarian Endometrial Stromal Cells and Their Expression of SF-1, ERβ, and Aromatase.

Applications

Unspecified application

Species

Unspecified reactive species

XiaoQing Wang,PeiLi Wu,Xin Li,Cheng Zeng,JingWen Zhu,YingFang Zhou,Ye Lu,Qing Xue

Molecular and cellular biology 41:e0035721 PubMed34460330

2021

Regulation of Estradiol Synthesis by Aromatase Interacting Partner in Breast (AIPB).

Applications

Unspecified application

Species

Unspecified reactive species

Himangshu S Bose,Randy M Whittal,Curtis E Lanier,Brendan Marshall,Maheshinie Rajapaksha,Brian W Wheeler,Nicholas D Carbo,Elin M Hahn,Elizabeth W Perry,Neal M Hall,Mikhail M Melomed,Edward L Perkins,William E Burak

Oncology letters 16:4577-4585 PubMed30214592

2018

Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes invasion in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jieqi Ke,Zhen Shen,Min Li,Cheng Peng,Ping Xu,Meimei Wang,Yi Zhu,Xuefen Zhang,Dabao Wu

Journal of inorganic biochemistry 184:79-87 PubMed29684698

2018

Biophysical characterization of Aptenodytes forsteri cytochrome P450 aromatase.

Applications

Species

Unspecified reactive species

Francisco Zarate-Perez,Jesús B Velázquez-Fernández,Gareth K Jennings,Lisa S Shock,Charles E Lyons,John C Hackett

Scientific reports 7:17289 PubMed29229929

2017

Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer.

Applications

IHC-P

Species

Unspecified reactive species

Sreenivas Adurthi,Mahesh M Kumar,H S Vinodkumar,Geetashree Mukherjee,H Krishnamurthy,K Kshitish Acharya,U D Bafna,Devi K Uma,B Abhishekh,Sudhir Krishna,A Parchure,Murali Alka,R S Jayshree

Fertility and sterility 106:673-80 PubMed27320036

2016

Aromatase inhibitor regulates let-7 expression and let-7f-induced cell migration in endometrial cells from women with endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

SiHyun Cho,Levent Mutlu,Yuping Zhou,Hugh S Taylor

Veterinary pathology 52:1045-51 PubMed25883120

2015

Effects of Obesity and Obesity-Related Molecules on Canine Mammary Gland Tumors.

Applications

IHC-P

Species

Dog

H-Y Lim,K-S Im,N-H Kim,H-W Kim,J-I Shin,J-Y Yhee,J-H Sur

Stem cells translational medicine 4:261-8 PubMed25666845

2015

Functional maintenance of differentiated embryoid bodies in microfluidic systems: a platform for personalized medicine.

Applications

ICC/IF

Species

Mouse

Sinan Guven,Jennifer S Lindsey,Ishwari Poudel,Sireesha Chinthala,Michael D Nickerson,Behzad Gerami-Naini,Umut A Gurkan,Raymond M Anchan,Utkan Demirci

Biology of reproduction 91:68 PubMed25078683

2014

The steroid hormone environment during primordial follicle formation in perinatal mouse ovaries.

Applications

Unspecified application

Species

Mouse

Sudipta Dutta,Connie J Mark-Kappeler,Patricia B Hoyer,Melissa E Pepling

View all publications

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Abcam product promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-Aromatase 抗体

Anti-Aromatase antibody

Western blot - Anti-Aromatase antibody (AB35604)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (16)

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