AB124690

Anti-ARL4A 抗体 [EPR6086]

Anti-ARL4A antibody [EPR6086]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ARL4A antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

ARL4, ARL4A, ADP-ribosylation factor-like protein 4A

2 Images

Lab

Western blot - Anti-ARL4A antibody [EPR6086] (AB124690)

We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

Blocking/Diluting Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-ARL4A antibody [EPR6086] (ab124690) at 1/1000 dilution

All lanes:

Human small intestine tissue lysate prepared in 1% SDS Hot lysis method at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

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OI-RD Scanning - Anti-ARL4A antibody [EPR6086] (AB124690)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ARL4A antibody [EPR6086] (AB124690)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR6086

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ARL4A (ADP-Ribosylation Factor-Like 4A) acts as a small GTPase in the ADP-ribosylation factor family. This protein also known as ARF-like protein has a mass of approximately 20 kDa. ARL4A is expressed in various tissues including the brain and kidneys. It plays an important role in intracellular signaling impacting cytoskeleton dynamics and membrane trafficking.

Biological function summary

ARL4A impacts cellular processes through its involvement in actin cytoskeleton reorganization. This makes ARL4A important in cell migration and morphogenesis. As part of the ARF family ARL4A may interact with other small GTPases forming a signaling complex that modulates cellular function. Its activity helps maintain cell structure and movement reflecting its influence on morphogenetic processes.

Pathways

ARL4A participates in signal transduction networks like the Ras-related pathways. It links to proteins such as ARF6 showing its involvement in vesicular trafficking and cytoskeleton remodeling. Through these pathways ARL4A helps coordinate intracellular activities that maintain cell shape and adhesion contributing to overall cellular dynamics.

ARL4A links to pathological conditions like cancer and metabolic disorders. Abnormal ARL4A expression associates with oncogenic processes suggesting its influence on tumorigenesis. Additionally disruptions in ARL4A function might involve interactions with proteins like ARL6 indicating potential metabolic dysregulation. These connections highlight ARL4A's significance in disease development and progression marking it as an important target in disease research.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form.

See full target information ARL4A

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

MedComm 6:e70148 PubMed40151835

2025

Transforming Growth Factor Beta2 Promotes Migration and Inhibits the Proliferation of Gastric Cancer Cells by Regulating the pSmad2/3-NDRG1 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feng-Jun He,Xiao-Long Chen,Yun-Feng Zhu,Hua-Yang Pang,Ze-Dong Li,Pan-Ping Liang,Tao Jin,Zheng-Wen Chen,Ze-Hua Chen,Jian-Kun Hu,Kun Yang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARL4A 抗体 [EPR6086]

Anti-ARL4A antibody [EPR6086]

Western blot - Anti-ARL4A antibody [EPR6086] (AB124690)

OI-RD Scanning - Anti-ARL4A antibody [EPR6086] (AB124690)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise