AB178425

Anti-ARL15 抗体 [EPR12591]

Anti-ARL15 antibody [EPR12591]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal ARL15 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

別名を表示する

ARFRP2, ARL15, ADP-ribosylation factor-like protein 15, ADP-ribosylation factor-related protein 2, ARF-related protein 2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-ARL15 antibody [EPR12591] (AB178425)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARL15 antibody [EPR12591] (AB178425)

Immunofluorescent analysis of K562 cells labeling ARL15 with ab178425 at 1/50 dilution (red). DAPI nuclear staining (blue).

Supplier Data

Immunoprecipitation - Anti-ARL15 antibody [EPR12591] (AB178425)

Western blot analysis on immunoprecipitation pellet from (1) Human fetal kidney lysate or (2) 1X PBS (negative control) immunoprecipitated using ab178425 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-ARL15 antibody [EPR12591] (ab178425)

Predicted band size: 22 kDa

false

Supplier Data

Western blot - Anti-ARL15 antibody [EPR12591] (AB178425)

All lanes:

Western blot - Anti-ARL15 antibody [EPR12591] (ab178425) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

K562 lysate at 10 µg

Lane 3:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 22 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR12591

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IP, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ARL15 also known as ADP-ribosylation factor-like protein 15 is a member of the ARF family of GTPases. This small GTP-binding protein weighs about 21 kDa. ARL15 resides mainly in the cytoplasm but its expression is also notable in adipose tissue and the pancreas. Mechanical interaction of ARL15 with membranes and other molecules is characteristic for proteins in the ARF family indicating a role in cellular functions that involve vesicle trafficking or membrane remodeling.

Biological function summary

ARL15 influences metabolic processes and signaling pathways. It integrates into multiprotein complexes that regulate various cellular activities. Research suggests its involvement in lipid metabolism and adipogenesis playing a role in the regulation of insulin sensitivity. These biological functions make ARL15 significant for maintaining cellular energy balance and metabolic homeostasis.

Pathways

ARL15 engages with two central pathways: the insulin signaling pathway and lipid metabolism. It influences these pathways by interacting with key proteins such as IRS1 in insulin signaling which impacts glucose uptake and energy regulation. Additionally in lipid metabolism ARL15 associates with proteins that control fatty acid oxidation and storage contributing to the overall lipid balance within the cell.

ARL15 has connections to type 2 diabetes and obesity. People with altered expression levels of ARL15 often exhibit changes in insulin sensitivity linking it to the pathophysiology of type 2 diabetes. ARL15 also interacts with PPARγ a protein highly involved in fat tissue development and implicated in obesity. These interactions indicate potential pathways through which ARL15 influences metabolic conditions making it a target of interest for disease research.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information ARL15

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23211 PubMed37773757

2023

Cleft palate and minor metabolic disturbances in a mouse global Arl15 gene knockout.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Bai,Liz Bentley,Chao Ma,Navaratnam Naveenan,James Cleak,Yixing Wu,Michelle M Simon,Henrik Westerberg,Ramón Casero Cañas,Neil Horner,Rajesh Pandey,Keanu Paphiti,Ulrike Schulze,Joffrey Mianné,Tertius Hough,Lydia Teboul,Jeroen H F de Baaij,Roger D Cox

Biology open 10: PubMed34779483

2021

Palmitoylated small GTPase ARL15 is translocated within Golgi network during adipogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yixing Wu,Ying Bai,David G McEwan,Liz Bentley,Dimitra Aravani,Roger D Cox

Theranostics 11:6461-6476 PubMed33995668

2021

Single-cell transcriptome profiling reveals vascular endothelial cell heterogeneity in human skin.

Applications

Unspecified application

Species

Unspecified reactive species

Qingyang Li,Zhenlai Zhu,Lei Wang,Yiting Lin,Hui Fang,Jie Lei,Tianyu Cao,Gang Wang,Erle Dang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARL15 抗体 [EPR12591]

Anti-ARL15 antibody [EPR12591]

Immunocytochemistry/ Immunofluorescence - Anti-ARL15 antibody [EPR12591] (AB178425)

Immunoprecipitation - Anti-ARL15 antibody [EPR12591] (AB178425)

Western blot - Anti-ARL15 antibody [EPR12591] (AB178425)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Proteins & Peptides

Abcam product promise