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AB226776

Anti-ARID5B 抗体

Anti-ARID5B antibody

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(1 Publication)

Rabbit Polyclonal ARID5B antibody. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARID5B.

別名を表示する

DESRT, MRF2, ARID5B, AT-rich interactive domain-containing protein 5B, ARID domain-containing protein 5B, MRF1-like protein, Modulator recognition factor 2, MRF-2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ARID5B antibody (AB226776)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARID5B antibody (AB226776)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling ARID5B protein at nucleus using ab226776 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID5B antibody (AB226776)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID5B antibody (AB226776)

Immunohistochemical analysis of mouse intestine tissue stained for ARID5B protein at nucleus with ab226776 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID5B antibody (AB226776)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID5B antibody (AB226776)

Immunohistochemical analysis of mouse intestine tissue stained for ARID5B protein at nucleus with ab226776 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-ARID5B antibody (AB226776)
  • WB

Supplier Data

Western blot - Anti-ARID5B antibody (AB226776)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-ARID5B antibody (ab226776) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract at 30 µg

Predicted band size: 132 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Mouse

アプリケーション

WB, ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human ARID5B. The exact immunogen used to generate this antibody is proprietary information.

Q14865

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ARID5B also known as AT-rich interactive domain-containing protein 5B functions as a transcription factor. This protein with a mass of approximately 132 kilodaltons interacts with DNA to regulate gene expression by binding to specific AT-rich sequences. ARID5B localizes in the nucleus and exhibits expression in various tissues including the lungs spleen and liver highlighting its involvement in multiple biological processes.
Biological function summary

The activities of AT-rich sequences within the gene regulatory network depend significantly on transcription factors. ARID5B plays an important role in modulating the expression of genes involved in cell differentiation and development. It functions within a complex of proteins that participate in chromatin remodeling which alters the accessibility of chromatin to the transcriptional machinery. Through these interactions ARID5B ensures precise control over gene expression necessary for proper cellular function and organism development.

Pathways

Transcription factors like ARID5B integrate signals from various regulatory networks. ARID5B engages in the Wnt signaling and Notch pathways influencing stem cell differentiation and maintaining tissue homeostasis. In these pathways ARID5B interacts with other proteins like TCF/LEF transcription factors in the Wnt pathway and NICD (Notch intracellular domain) in the Notch pathway coordinating the transcriptional response required for cell fate determination.

ARID5B mutations or dysregulation have associations with certain types of cancer and autoimmune diseases. Acute lymphoblastic leukemia (ALL) shows links to altered ARID5B function contributing to abnormal cell proliferation. Additionally variations in ARID5B influence the risk of developing type 1 diabetes where it interacts with other immune-related proteins such as HLA-DRB1. Researchers study these interactions to understand disease mechanisms and explore potential therapeutic strategies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis : regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer.
See full target information ARID5B
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Oncogenesis 7:84 PubMed30420689

2018

Aberrant ARID5B expression and its association with Ikaros dysfunction in acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Ge,Qi Han,Yan Gu,Qinyu Ge,Jinlong Ma,Justin Sloane,Guofeng Gao,Kimberly J Payne,Laszlo Szekely,Chunhua Song,Sinisa Dovat
View all publications
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