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AB314046

Anti-ARID2 抗体 [BLR213K] - BSA free

Anti-ARID2 antibody [BLR213K] - BSA free

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Rabbit Recombinant Monoclonal ARID2 antibody. Suitable for Flow Cyt (Intra), ICC, IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ARID2 aa 1750 to C-terminus.

別名を表示する

BAF200, KIAA1557, ARID2, AT-rich interactive domain-containing protein 2, ARID domain-containing protein 2, BRG1-associated factor 200, Zinc finger protein with activation potential, Zipzap/p200

4 Images
Flow Cytometry (Intracellular) - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)

Detection of human ARID2 (shaded) in Jurkat cells by flow cytometry. Antibody : Rabbit anti-ARID2 recombinant monoclonal antibody [BLR0213K] (ab314046) or isotype control (unshaded). Secondary : DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

Immunocytochemistry - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)
  • ICC

Supplier Data

Immunocytochemistry - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)

Sample : FFPE section of LNCaP (Human prostate cancer cell line) cells. Antibody : Standard version of ab314046 at a 1/100 dilution. Secondary : HRP-conjugated goat anti-rabbit IgG. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE cell sections.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)

This data was developed using the standard version of ab314046, the same antibody clone in a different buffer formulation. Sample : FFPE section of human prostate carcinoma. Antibody : Standard version of ab314046 at a 1/100 dilution. Secondary : HRP-conjugated goat anti-rabbit IgG. Epitope retrieval with Tris-EDTA pH6.0 is recommended for FFPE tissue sections.

Western blot - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)
  • WB

Supplier Data

Western blot - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)

Samples : Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, MOLT-4, and SK-N-BE(2) cells prepared using NETN lysis buffer. Antibody : Rabbit anti-ARID2 recombinant monoclonal antibody [BLR213K] (A700-213 lot 1) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 75 seconds. Lower Panel : Rabbit anti-COPB2 antibody (A304-523A).

All lanes:

Western blot - Anti-ARID2 antibody [BLR213K] - BSA free (ab314046) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

Lane 4:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

Lane 5:

SK-N-BE(2) (Human neuroblastoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

BLR213K

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IHC-P, ICC, Flow Cyt (Intra)

applications

免疫原

Synthetic Peptide within Human ARID2 aa 1750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q68CP9

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Reactivity data

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製品の詳細

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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出荷温度及び保存条件

製品の状態
Liquid
バッファー組成
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline
出荷温度
Blue Ice
短期保存期間
Up to 12 months
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ARID2 also known as AT-rich interactive domain-containing protein 2 is a component of the SWI/SNF chromatin remodeling complex. It has a molecular mass of approximately 183 kDa. ARID2 is expressed in various tissues with high levels found in the liver kidney and testis. In the cell nucleus it functions as a DNA-binding protein playing a role in modulating chromatin structure through its interaction with other chromatin-associated proteins.
Biological function summary

ARID2 contributes to regulating gene expression by remodeling chromatin allowing transcription factors access to DNA. It acts as a part of the PBAF subtype of the SWI/SNF complex which includes proteins like BRG1 and BAF200. This complex influences transcriptional activation and repression aiding the regulation of genes important for cellular differentiation and development. Loss or mutation of ARID2 affects normal biological processes indicating its role in ensuring proper cellular function.

Pathways

ARID2 plays a role in the RAS signaling and the Hedgehog signaling pathways. It interacts with proteins such as BAF155 and BAF170 which are essential for recruiting and assembling the PBAF complex on chromatin. These pathways are critical for cellular proliferation and differentiation ensuring that cells respond accurately to signals which is vital for maintaining tissue homeostasis and proper organismal development.

ARID2 mutations are linked to hepatocellular carcinoma and malignant melanoma. These mutations are associated with abnormalities in chromatin remodeling that lead to transcriptional dysregulation. The target is also connected to proteins like BRD9 and BRD7 within the context of cancer which are involved in tumor suppression. Alterations in ARID2 might contribute to the progression and aggressiveness of these cancers by disrupting normal chromatin dynamics and gene expression patterns.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes.
See full target information ARID2
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