Anti-ARID2 抗体 (ab245529)
Key features and details
- Rabbit polyclonal to ARID2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ARID2 antibody
ARID2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ARID2 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human ARID2 aa 1600-1650. The exact sequence is proprietary. NP_689854.2
Database link: Q68CP9 -
ポジティブ･コントロール
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245529 was affinity purified using an epitope specific to ARID2 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245529の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 5-10 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 197 kDa.
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| 特記事項 |
|---|
|
IP
Use at 5-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 197 kDa. |
ターゲット情報
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機能
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes. -
組織特異性
Highly expressed in heart and testis. -
配列類似性
Contains 1 ARID domain.
Contains 1 C2H2-type zinc finger.
Contains 1 RFX-type winged-helix DNA-binding domain. -
発生段階
Up-regulated in adult heart (at protein level). -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 196528 Human
- Omim: 609539 Human
- SwissProt: Q68CP9 Human
- Unigene: 317304 Human
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別名
- ARID domain containing protein 2 antibody
- ARID domain-containing protein 2 antibody
- ARID2 antibody
see all
画像
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Western blot - Anti-ARID2 antibody (ab245529)All lanes : Anti-ARID2 antibody (ab245529) at 1/2000 dilution
Lane 1 : Wild-type HeLa cell lysate at 20 µg
Lane 2 : ARID2 knockout HeLa cell lysate at 20 µg
Lane 3 : HEK-293 cell lysate at 10 µg
Lane 4 : U-87 MG cell lysate at 10 µg
Performed under reducing conditions.
Predicted band size: 197 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-ARID2 antibody staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab245529 was shown to bind specifically to ARID2. A band was observed at 220 kDa in wild-type HeLa cell lysates with no signal observed at this size in ARID2 knockout cell line ab265137 (knockout cell lysate ab257357). To generate this image, wild-type and ARID2 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Western blot - Anti-ARID2 antibody (ab245529)All lanes : Anti-ARID2 antibody (ab245529) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 197 kDa
Exposure time: 3 minutesLysates prepared in NETN buffer.
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Immunoprecipitation - Anti-ARID2 antibody (ab245529)ARID2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245529 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab245529 at 1 µg/ml.
Lane 1: ab245529 (Batch 1) IP in HeLa whole cell lysate.
Lane 2: ab245529 (Batch 2) IP in HeLa whole cell lysate.
Lane 3: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Lysates prepared in NETN buffer.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245529 は論文での使用が確認できていません。