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Anti-ARID1B 抗体 [2D2] (ab57461)

Reviews (2)Q&A (2)References (28)
Western blot - Anti-ARID1B antibody [2D2] (ab57461)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1B antibody [2D2] (ab57461)
  • Immunocytochemistry/ Immunofluorescence - Anti-ARID1B antibody [2D2] (ab57461)
  • Sandwich ELISA - Anti-ARID1B antibody [2D2] (ab57461)

Key features and details

  • Mouse monoclonal [2D2] to ARID1B
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2b

製品の概要

  • 製品名

    Anti-ARID1B antibody [2D2]
    ARID1B 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [2D2] to ARID1B

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment: PPAKRHEGDM YNMQYSSQQQ EMYNQYGGSY SGPDRRPIQG QYPYPYSRER MQGPGQIQTH GIPPQMMGGP LQSSSSEGPQ QNMWAARNDM PYPYQNR, corresponding to amino acids 1364-1461 of Human ARID1B

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • ICC/IF: HepG2 cells.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 12 Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab57461の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 236 kDa.
IHC-P (1)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 236 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性

    ARID1B (AT-rich interactive domain-containing protein 1B) is a component of SWI/SNF chromatin remodeling complexes involved in transcriptional activation and repression of select genes by chromatin remodeling. It binds DNA non-specifically and alters DNA-nucleosome topology.

  • 細胞内局在

    Nuclear

  • 参照データベース

    • 別名

      • 6A3 5 antibody
      • ARID 1B antibody
      • ARID domain containing protein 1B antibody
      • AT rich interactive domain 1B (SWI1 like) antibody
      • AT rich interactive domain 1B antibody
      • AT rich interactive domain containing protein 1B antibody
      • BAF 250b antibody
      • BAF-associated factor, 250-KD, B antibody
      • BAF250b antibody
      • BRG1 associated factor 250b antibody
      • BRG1 binding protein antibody
      • BRG1 binding protein ELD/OSA1 antibody
      • BRG1 binding protein hELD/OSA1 antibody
      • BRIGHT antibody
      • DAN 15 antibody
      • DAN15 antibody
      • Eld (eyelid)/Osa protein antibody
      • ELD/OSA1 antibody
      • hELD/OSA1 antibody
      • hOsa 2 antibody
      • hOsa2 antibody
      • KIAA1235 antibody
      • MRD12 antibody
      • OSA 2 antibody
      • Osa homolog 2 antibody
      • OSA2 antibody
      • OTTHUMP00000040115 antibody
      • p250R antibody
      • RP11 419L10.1 antibody
      see all

    画像

    • Western blot - Anti-ARID1B antibody [2D2] (ab57461)
      Western blot - Anti-ARID1B antibody [2D2] (ab57461)
      Anti-ARID1B antibody [2D2] (ab57461) at 5 µg/ml + HeLa (human cervix adenocarcinoma epithelial cell) Nuclear Extract at 50 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size: 236 kDa



      Blocking and diluting buffer and concentration: 5% non-fat milk/PBST (0.2%).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1B antibody [2D2] (ab57461)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1B antibody [2D2] (ab57461)

      Immunoperoxidase analysis of formalin-fixed paraffin-embedded human kidney tissue labelling ARID1B with ab129165 at 3 µg/mL concentration. 

    • Immunocytochemistry/ Immunofluorescence - Anti-ARID1B antibody [2D2] (ab57461)
      Immunocytochemistry/ Immunofluorescence - Anti-ARID1B antibody [2D2] (ab57461)

      Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labelling ARID1B (Green) with ab129165 at 10 µg/mL concentration.

    • Sandwich ELISA - Anti-ARID1B antibody [2D2] (ab57461)
      Sandwich ELISA - Anti-ARID1B antibody [2D2] (ab57461)
      Sandwich ELSIA standard curve using ab57461 as a capture antibody. Detection limit for recombinant GST tagged ARID1B is 0.03 ng/mL.

    参考文献 (28)

    ab57461 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab57461 は 28 報の論文で使用されています。

    • Vital T  et al. MS0621, a novel small-molecule modulator of Ewing sarcoma chromatin accessibility, interacts with an RNA-associated macromolecular complex and influences RNA splicing. Front Oncol 13:1099550 (2023). PubMed: 36793594
    • Reddy D  et al. Paraspeckles interact with SWI/SNF subunit ARID1B to regulate transcription and splicing. EMBO Rep 24:e55345 (2023). PubMed: 36354291
    • Belk JA  et al. Genome-wide CRISPR screens of T cell exhaustion identify chromatin remodeling factors that limit T cell persistence. Cancer Cell 40:768-786.e7 (2022). PubMed: 35750052
    • Kim H  et al. Early postnatal serotonin modulation prevents adult-stage deficits in Arid1b-deficient mice through synaptic transcriptional reprogramming. Nat Commun 13:5051 (2022). PubMed: 36030255
    • Moffat JJ  et al. Differential roles of ARID1B in excitatory and inhibitory neural progenitors in the developing cortex. Sci Rep 11:3856 (2021). PubMed: 33594090
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ARID1B antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA pH8.5
    Permeabilization
    No
    Specification
    kidney
    Blocking step
    Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Sep 07 2015

    Western blot abreview for Anti-ARID1B antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (JHH4)
    Loading amount
    10 µg
    Specification
    JHH4
    Treatment
    Use nuclear fraction or cytoplasmic fraction
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 20 2011

    Question

    The customer has requested a credit for this antibody as he could not find a suitable replacement, he said;

    ARID1B is a nuclear protein and no doubt sonication would probably denature the protein. However, the protein would be denatured for Western blot anyway and would not matter. Furthermore, sonication would probably dissociate the potential interaction between ARID1B and genomic DNA that could potentially affect the run of SDS-PAGE (in my experience). Sonication was done immediately after cell harvest in high SDS and EDTA concentrations to prevent any potential rapid proteolysis of ARID1B.

    Kind regards,

    Read More
    Answer

    Thank you for contacting us.

    Your credit note ID is 23074.

    I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

    (1) Redeemed against the original invoice if this hasn't already been paid.
    (2) Held on the account for use against a future order.
    (3) A full refund can be offered where no other invoices are outstanding.

    Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

    To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

    The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

    Read More

    Question

    Lot number: GR94192-1
    Inquiry: GENERAL INFORMATION Antibody Storage Conditions (temperature/reconstitution etc) As instructed. -20°C and freeze/thaw was avoided Description of the Problem (high background, wrong band size, more bands, no bands etc) Wrong band size. 2 non-specific bands between 52kDa and 30kDa Sample (Species/Cell extract/Purified protein/Recombinant protein, etc) HEK293FT, BE2-M17 neuroblastoma and human fibroblasts lysates. Sample Preparation (Buffer/Protease inhibitors/Heating sample etc.) Lysed immediately with 50mM Tris, pH 6.8, 5% SDS, 50mM EDTA on the culture plate. Sample was then sonicated to shear genomic DNA. Protein concentration was measured and appropriate amount was then mixed with the required volume of 5X SDS-PAGE sample loading buffer Amount of protein loaded 80ug Electrophoresis/Gel Conditions (Reducing or non-reducing gel, % of the gel etc.) Reducing, 10% polyacrylamide Transfer and blocking conditions (Buffer/time period, Blocking agent etc) 10v, overnight wet transfer for 16.5 hrs. Blocking agent, 5% BSA in TBST, for 30min Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, wash step) Abcam/ Host species: mouse target species: human/ diluents: 1% BSA in TBST/ Dilution 1:500/ incubation time: overnight at 4°C/ Wash: 3x 5min wash with TBST Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, wash step) Manufacturer: Dako/ Species: rabbit/ Diluent: 1% BSA in TBST/ Dilution: 1:8000/ Incubation time: 1hr/ Wash: 3x 5min wash with TBST Detection Method (ECL, ECL Plus etc.) ECL Plus Positive and negative controls used (please specify) No available positive control Plausible negative control: Patient fibroblast with only one copy of ARID1B OPTIMIZATION ATTEMPTS (PROBLEM SOLVING) How many times have you tried the Western Blot? 5 Have you run a “No Primary” control? Yes Do you obtain the same results every time? Yes e.g. are the background bands always in the same place? What steps have you altered? Extending incubation time, and trying other cell lysates Comments from Sapphire: Sapphire Order #:PO-15546 Abcam Invoice #: 1350925B Not sure of exact lot # but this is the first time we have ordered this product.

    Read More
    Answer

    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

    I have read the details you have kindly provided and have following further questions for better understanding of the problem;

    - Could you confirm if the immunoprecipitation has been done or not?
    - Have you used any loading control?

    Suggestions for further improvements;

    -Could you try the lysates without sonicating?

    Thank you very much for your cooperation. I will look forward to hearing from you soon.

    Read More

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