Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-ARID1A 抗体 [EPR24489-2] (BSA and Azide free - BSA and Azide free (Detector) (ab302736)

Submit a review Submit a question
Sandwich ELISA - Anti-ARID1A antibody [EPR24489-2] (BSA and Azide free - BSA and Azide free (Detector) (ab302736)
  • Anti-ARID1A antibody [EPR24489-2] (BSA and Azide free (Detector)) (AB302736)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24489-2] to ARID1A - BSA and Azide free (Detector)
  • Suitable for: Sandwich ELISA
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-ARID1A antibody [EPR24489-2] (BSA and Azide free - BSA and Azide free (Detector)
    ARID1A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24489-2] to ARID1A - BSA and Azide free (Detector)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    ab302735 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ARID1A. The recommended pair for sandwich ELISA is:
         Capture: ab302735, ARID1A Capture Antibody (unconjugated)
         Detector: ab302736, ARID1A Detector Antibody (unconjugated)
    The reference range value is 1.72 - 110 ng/ml.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302736の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR24489-1] to ARID1A - BSA and Azide free (Capture) (ab302735).
特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR24489-1] to ARID1A - BSA and Azide free (Capture) (ab302735).

ターゲット情報

  • 機能

    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Binds DNA non-specifically. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.

  • 組織特異性

    Highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and PBL, and at a much lower level in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.

  • 配列類似性

    Contains 1 ARID domain.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • actin-dependent regulator of chromatin subfamily F member 1 antibody
      • ARI1A_HUMAN antibody
      • ARID domain containing protein 1A antibody
      • ARID domain-containing protein 1A antibody
      • ARID1A antibody
      • AT rich interactive domain 1A (SWI like) antibody
      • AT rich interactive domain 1A antibody
      • AT rich interactive domain containing protein 1A antibody
      • AT-rich interactive domain-containing protein 1A antibody
      • B120 antibody
      • BAF250 antibody
      • BAF250A antibody
      • BM029 antibody
      • brain protein 120 antibody
      • BRG1 associated factor 250 antibody
      • BRG1 associated factor 250a antibody
      • BRG1-associated factor 250 antibody
      • BRG1-associated factor 250a antibody
      • C1ORF4 antibody
      • chromatin remodeling factor p250 antibody
      • chromosome 1 open reading frame 4 antibody
      • ELD antibody
      • hELD antibody
      • hOSA1 antibody
      • matrix-associated antibody
      • MRD14 antibody
      • Osa homolog 1 antibody
      • OSA1 antibody
      • OSA1 nuclear protein antibody
      • P270 antibody
      • SMARCF1 antibody
      • SWI like protein antibody
      • SWI SNF complex protein p270 antibody
      • SWI-like protein antibody
      • SWI/SNF complex protein p270 antibody
      • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily f, member 1 antibody
      • SWI/SNF-related antibody
      see all

    画像

    • Sandwich ELISA - Anti-ARID1A antibody [EPR24489-2] (BSA and Azide free - BSA and Azide free (Detector) (ab302736)
      Sandwich ELISA - Anti-ARID1A antibody [EPR24489-2] (BSA and Azide free - BSA and Azide free (Detector) (ab302736)
      Sandwich ELISA of ab302734 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL
    • Anti-ARID1A antibody [EPR24489-2] (BSA and Azide free (Detector)) (AB302736)
      Anti-ARID1A antibody [EPR24489-2] (BSA and Azide free (Detector)) (AB302736)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302736 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302736 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab302736.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com