Anti-ARHGAP29 抗体 (ab85853)
Key features and details
- Rabbit polyclonal to ARHGAP29
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ARHGAP29 antibody -
製品の詳細
Rabbit polyclonal to ARHGAP29 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human ARHGAP29 aa 1211-1261.
Database link: NP_004806.3 -
ポジティブ･コントロール
- HeLa, 293T and NIH3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab85853 was affinity purified using an epitope specific to ARHGAP29 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85853の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 142 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 142 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho. In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis. -
組織特異性
Widely expressed. Highly expressed in skeletal muscle and heart. Expressed at intermediate level in placenta, liver and pancreas. Weakly expressed in brain, lung and kidney. -
配列類似性
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Rho-GAP domain. - Information by UniProt
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参照データベース
- Entrez Gene: 9411 Human
- Entrez Gene: 214137 Mouse
- Omim: 610496 Human
- SwissProt: Q52LW3 Human
- SwissProt: Q8CGF1 Mouse
- Unigene: 483238 Human
- Unigene: 229287 Mouse
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別名
- ARHGAP 29 antibody
- Arhgap29 antibody
- ARHGAP29 protein antibody
see all
画像
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Western blot - Anti-ARHGAP29 antibody (ab85853)All lanes : Anti-ARHGAP29 antibody (ab85853) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 142 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Immunoprecipitation - Anti-ARHGAP29 antibody (ab85853)Detection of ARHGAP29 by Western Blot of Immunprecipitate.
ab85853, at 1 µg/ml, staining ARHGAP29 in HeLa whole cell lysate immunoprecipitated using ab85853 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab85853 は 2 報の論文で使用されています。
- Shimizu K et al. ARHGAP29 expression may be a novel prognostic factor of cell proliferation and invasion in prostate cancer. Oncol Rep 44:2735-2745 (2020). PubMed: 33125156
- Hernández CJ et al. PINK1 Silencing Modifies Dendritic Spine Dynamics of Mouse Hippocampal Neurons. J Mol Neurosci 69:570-579 (2019). PubMed: 31486971