Anti-ARHGAP27 抗体 [EPR12250] (ab171973)
![Western blot - Anti-ARHGAP27 antibody [EPR12250] (ab171973)](https://www.abcam.co.jp/ps/products/171/ab171973/Images/ab171973-194481-5.jpg "Western blot - Anti-ARHGAP27 antibody [EPR12250] (ab171973)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12250] to ARHGAP27
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ARHGAP27 antibody [EPR12250]
ARHGAP27 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12250] to ARHGAP27 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
適用なし: IP -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Human ARHGAP27 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q6ZUM4 -
ポジティブ･コントロール
- HepG2, A549 and BxPC-3 cell lysates; Human breast carcinoma tissue; Human pancreas tissue; BxPC-3 cells and permeabilized A549 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12250 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab171973の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 98 kDa.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/50 - 1/100.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 98 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/50 - 1/100. |
ターゲット情報
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機能
Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1. -
組織特異性
Expressed in germinal center B-cell, spleen, chronic lymphocytic leukemia, pancreatic cancer and lung cancer. -
配列類似性
Contains 1 PH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain.
Contains 3 WW domains. -
細胞内局在
Cytoplasm. Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 201176 Human
- Omim: 610591 Human
- SwissProt: Q6ZUM4 Human
- Unigene: 205326 Human
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別名
- ARHGAP27 antibody
- CAMGAP1 antibody
- CIN85-associated multi-domain-containing Rho GTPase-activating protein 1 antibody
see all
画像
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Western blot - Anti-ARHGAP27 antibody [EPR12250] (ab171973)All lanes : Anti-ARHGAP27 antibody [EPR12250] (ab171973) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 98 kDaNote: primary antibody diluted in BSA.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] (ab171973)](https://www.abcam.co.jp/ps/products/171/ab171973/Images/ab171973-194479-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] (ab171973)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ARHGAP27 with ab171973 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] (ab171973)](https://www.abcam.co.jp/ps/products/171/ab171973/Images/ab171973-194480-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP27 antibody [EPR12250] (ab171973)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling ARHGAP27 with ab171973 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP27 antibody [EPR12250] (ab171973)](https://www.abcam.co.jp/ps/products/171/ab171973/Images/ab171973-194478-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP27 antibody [EPR12250] (ab171973)Immunofluorescent analysis of BxPC-3 cells labeling ARHGAP27 with ab171973 (green) at 1/50 dilution. DAPI nuclear staining (blue).
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![Flow Cytometry (Intracellular) - Anti-ARHGAP27 antibody [EPR12250] (ab171973)](https://www.abcam.co.jp/ps/products/171/ab171973/Images/ab171973-194477-1.jpg)
Flow Cytometry (Intracellular) - Anti-ARHGAP27 antibody [EPR12250] (ab171973)Intracellular flow cytometric analysis of permeabilized A549 cells labeling ARHGAP27 with ab171973 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).
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![Anti-ARHGAP27 antibody [EPR12250] (ab171973)](https://www.abcam.co.jp/ps/products/171/ab171973/Images/ab171973-6-benefits-of-recombinant-antibodies.png)
Anti-ARHGAP27 antibody [EPR12250] (ab171973)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab171973 は論文での使用が確認できていません。