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AB224751

Anti-ARH3 抗体

Anti-ARH3 antibody

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(1 Publication)

Rabbit Polyclonal ARH3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ADPRS aa 1-150.

別名を表示する

ADPRHL2, ARH3, ADPRS, ADP-ribosylhydrolase ARH3, ADP-ribose glycohydrolase ARH3, ADP-ribosylhydrolase 3, O-acetyl-ADP-ribose deacetylase ARH3, Poly(ADP-ribose) glycohydrolase ARH3, [Protein ADP-ribosylarginine] hydrolase-like protein 2, [Protein ADP-ribosylserine] hydrolase

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ARH3 antibody (AB224751)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARH3 antibody (AB224751)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ARH3 (green) using ab224751 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARH3 antibody (AB224751)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARH3 antibody (AB224751)

Paraffin-embedded human Fallopian tube tissue stained for ARH3 using ab224751 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARH3 antibody (AB224751)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARH3 antibody (AB224751)

Paraffin-embedded human testis tissue stained for ARH3 using ab224751 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-ARH3 antibody (AB224751)
  • WB

Supplier Data

Western blot - Anti-ARH3 antibody (AB224751)

All lanes:

Western blot - Anti-ARH3 antibody (ab224751) at 1/100 dilution

Lane 1:

Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

ARH3 overexpression HEK-293T cell lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)

Predicted band size: 39 kDa

true

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF, WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human ADPRS aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9NX46

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ARH3 also known as ADP-ribosylhydrolase 3 is an enzyme involved in the removal of ADP-ribose groups from proteins a process known as de-ADP-ribosylation. It has a mass of approximately 39 kDa. The enzyme shows widespread expression in human tissues with higher levels in the brain heart and skeletal muscles. This expression pattern indicates its important role in various cellular processes across different tissue types.
Biological function summary

ARH3 catalyzes the hydrolysis of ADP-ribose units attached to serine residues on target proteins reversing the actions of PARPs (poly ADP-ribose polymerases) especially PARP1. By doing this it plays a role in regulating the post-translational modification of proteins. Although not considered a part of a permanent complex ARH3 often interacts with other protein factors involved in DNA repair and cellular stress responses suggesting its dynamic involvement in cellular regulation.

Pathways

This enzyme functions in DNA repair and cellular stress response pathways. ARH3's activity complements the work of PARP1 by removing ADP-ribose groups added during the DNA damage response. This enzymatic balance is essential to prevent excessive ADP-ribosylation that can affect DNA repair processes. The interaction between ARH3 and PARP1 illustrates the coordinated efforts required to maintain genomic stability and respond to cellular stress.

Mutations or dysregulation of ARH3 are linked to neurodegenerative disorders and cancer. In neurological diseases proteins modified by ARH3-driven de-ADP-ribosylation may alter neuronal survival and function. Similarly in cancer improper ARH3 activity could lead to uncontrolled cell proliferation due to a faulty DNA damage response. Tumor protein 53 (TP53) might interact with ARH3 indicating potential pathways affected in cancerous conditions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

ADP-ribosylhydrolase that preferentially hydrolyzes the scissile alpha-O-linkage attached to the anomeric C1'' position of ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on serine and threonine, free poly(ADP-ribose) and O-acetyl-ADP-D-ribose (PubMed : 21498885, PubMed : 29907568, PubMed : 30045870, PubMed : 30401461, PubMed : 30830864, PubMed : 33186521, PubMed : 33769608, PubMed : 33894202, PubMed : 34019811, PubMed : 34321462, PubMed : 34479984, PubMed : 34625544). Specifically acts as a serine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to serine residues on proteins, thereby playing a key role in DNA damage response (PubMed : 28650317, PubMed : 29234005, PubMed : 30045870, PubMed : 33186521, PubMed : 34019811, PubMed : 34625544). Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed : 29480802, PubMed : 33186521, PubMed : 34625544). Does not hydrolyze ADP-ribosyl-arginine, -cysteine, -diphthamide, or -asparagine bonds (PubMed : 16278211, PubMed : 33769608). Also able to degrade protein free poly(ADP-ribose), which is synthesized in response to DNA damage : free poly(ADP-ribose) acts as a potent cell death signal and its degradation by ADPRHL2 protects cells from poly(ADP-ribose)-dependent cell death, a process named parthanatos (PubMed : 16278211). Also hydrolyzes free poly(ADP-ribose) in mitochondria (PubMed : 22433848). Specifically digests O-acetyl-ADP-D-ribose, a product of deacetylation reactions catalyzed by sirtuins (PubMed : 17075046, PubMed : 21498885). Specifically degrades 1''-O-acetyl-ADP-D-ribose isomer, rather than 2''-O-acetyl-ADP-D-ribose or 3''-O-acetyl-ADP-D-ribose isomers (PubMed : 21498885).
See full target information ADPRS
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 14:1124444 PubMed36814472

2023

Na and K transport and maturation stage ameloblast modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jake Ngu,Antonius L J J Bronckers,Kaitlin Katsura,Yan Zhang,Pamela K Den Besten
View all publications
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