AB195033

Anti-Arginine (glcnac ) 抗体 [EPR18251]

Anti-Arginine (glcnac ) antibody [EPR18251]

RabMAb
Recombinant
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(13 Publications)

Rabbit Recombinant Monoclonal Arginine antibody. Suitable for WB and reacts with Modified Amino Acid samples. Cited in 13 publications.

別名を表示する

L arginine

1 Images

Supplier Data

Western blot - Anti-Arginine (glcnac ) antibody [EPR18251] (AB195033)

5% NFDM/TBST : Blocking and dilution buffer.

The image was kindly provided by our collaborator Dr Feng Shao's lab, NIBS.

All lanes:

Western blot - Anti-Arginine (glcnac ) antibody [EPR18251] (ab195033) at 1/2000 dilution

Lane 1:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TRADD at 20 µg

Lane 2:

HEK-293 whole cell lysate transfected with TRADD and NleB at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR18251

アイソタイプ

IgG

キャリアフリー

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p>NleB contained an unprecedented N-acetylglucosamine (GlcNAc) transferase activity that specifically modified a conserved arginine in these death domains (Arg 235 in the TRADD death domain).</p>" } } }

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製品の詳細

This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Feng Shao, National Institute of Biological Sciences (NIBS), Beijing.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Arginine often referred to as ARG plays a central role in various biological processes. It is an amino acid with a molecular weight of about 174.2 Da. Arginine is expressed in multiple tissues including the liver and endothelium. It often serves as a precursor for the synthesis of various biomolecules such as nitric oxide. In scientific literature you may also encounter it under alternative spellings like 'arganine' or 'argenine' though these are less common.

Biological function summary

Arginine contributes to protein synthesis and serves as a substrate for enzymes like nitric oxide synthase. It forms part of larger protein complexes and participates in metabolic pathways. Nitric oxide produced from arginine acts as a signaling molecule that modulates vascular function by inducing vasodilation. It also takes part in immune responses and has a role in cell division wound healing and hormone release.

Pathways

Arginine plays a significant role in the urea cycle and the nitric oxide signaling pathway. It interacts with proteins such as ornithine transcarbamylase in the urea cycle facilitating the removal of ammonia. Meanwhile in the nitric oxide pathway it collaborates with nitric oxide synthase to produce nitric oxide which affects smooth muscle relaxation and neurotransmission.

Arginine has connections to cardiovascular diseases and immune-associated conditions. The production of nitric oxide which involves arginine impacts cardiovascular health and its dysregulation can lead to hypertension. Additionally arginine metabolism influences conditions like arginase deficiency which affects the urea cycle. These conditions can involve other proteins such as ornithine transcarbamylase demonstrating the interlinked nature of arginine with various biological and pathological processes.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Arginine

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2456:153-171 PubMed35612741

2022

Glycopeptide-Centric Approaches for the Characterization of Microbial Glycoproteomes.

Applications

Unspecified application

Species

Unspecified reactive species

Nichollas E Scott

Frontiers in microbiology 12:825743 PubMed35126337

2022

Arginine GlcNAcylation and Activity Regulation of PhoP by a Type III Secretion System Effector in .

Applications

Unspecified application

Species

Unspecified reactive species

Juan Xue,Yuxuan Huang,Hua Zhang,Jiaqingzi Hu,Xing Pan,Ting Peng,Jun Lv,Kun Meng,Shan Li

Frontiers in cellular and infection microbiology 10:419 PubMed32974215

2020

The Effector SseK3 Targets Small Rab GTPases.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyao Gan,Nichollas E Scott,Joshua P M Newson,Rachelia R Wibawa,Tania Wong Fok Lung,Georgina L Pollock,Garrett Z Ng,Ian van Driel,Jaclyn S Pearson,Elizabeth L Hartland,Cristina Giogha

Frontiers in cell and developmental biology 8:641 PubMed32766249

2020

Arg-GlcNAcylation on TRADD by NleB and SseK1 Is Crucial for Bacterial Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Xue,Shufan Hu,Yuxuan Huang,Qi Zhang,Xueying Yi,Xing Pan,Shan Li

Communications biology 3:287 PubMed32504010

2020

Arginine GlcNAcylation of Rab small GTPases by the pathogen Salmonella Typhimurium.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Meng,Xiaohui Zhuang,Ting Peng,Shufan Hu,Jin Yang,Zhen Wang,Jiaqi Fu,Juan Xue,Xing Pan,Jun Lv,Xiaoyun Liu,Feng Shao,Shan Li

Frontiers in cellular and infection microbiology 10:197 PubMed32432056

2020

Auto Arginine-GlcNAcylation Is Crucial for Bacterial Pathogens in Regulating Host Cell Death.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Xue,Xing Pan,Ting Peng,Meimei Duan,Lijie Du,Xiaohui Zhuang,Xiaobin Cai,Xueying Yi,Yang Fu,Shan Li

Scientific reports 10:1073 PubMed31974499

2020

An intra-bacterial activity for a T3SS effector.

Applications

Unspecified application

Species

Unspecified reactive species

Samir El Qaidi,Nichollas E Scott,Michael P Hays,Brian V Geisbrecht,Shelby Watkins,Philip R Hardwidge

Molecular cell 74:922-935.e6 PubMed30979585

2019

Structural and Functional Insights into Host Death Domains Inactivation by the Bacterial Arginine GlcNAcyltransferase Effector.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjin Ding,Xing Pan,Lijie Du,Qing Yao,Juan Xue,Hongwei Yao,Da-Cheng Wang,Shan Li,Feng Shao

Molecular & cellular proteomics : MCP 18:1138-1156 PubMed30902834

2019

Effectors SseK1 and SseK3 Target Death Domain Proteins in the TNF and TRAIL Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua P M Newson,Nichollas E Scott,Ivy Yeuk Wah Chung,Tania Wong Fok Lung,Cristina Giogha,Jiyao Gan,Nancy Wang,Richard A Strugnell,Nathaniel F Brown,Miroslaw Cygler,Jaclyn S Pearson,Elizabeth L Hartland

PLoS pathogens 14:e1007259 PubMed30125331

2018

A pathogen-derived effector modulates host glucose metabolism by arginine GlcNAcylation of HIF-1α protein.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxi Xu,Xing Liu,Huangyuan Zha,Sijia Fan,Dawei Zhang,Shan Li,Wuhan Xiao

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Arginine (glcnac ) 抗体 [EPR18251]

Anti-Arginine (glcnac ) antibody [EPR18251]

Western blot - Anti-Arginine (glcnac ) antibody [EPR18251] (AB195033)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

アプリケーション

免疫原

組合せ製品

Assay Kits

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (13)

Abcam product promise