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リコンビナントRabMAb

Anti-ARFRP1/ARP 抗体 [EPR3899] (ab108199)

Submit a review Submit a question References (3)
Western blot - Anti-ARFRP1/ARP antibody [EPR3899] (ab108199)
  • Immunocytochemistry/ Immunofluorescence - Anti-ARFRP1/ARP antibody [EPR3899] (ab108199)
  • Anti-ARFRP1/ARP antibody [EPR3899] (ab108199)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3899] to ARFRP1/ARP
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ARFRP1/ARP antibody [EPR3899]
    ARFRP1/ARP 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR3899] to ARFRP1/ARP

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details
    適用なし: Flow Cyt or IHC-P

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HepG2 and HEK-293T cell lysate. Fetal liver and ovary cancer lysates ICC/IF: HeLa cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR3899

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108199の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).
ICC/IF
1/500.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).
ICC/IF
1/500.
追加情報
Is unsuitable for Flow Cyt or IHC-P.

ターゲット情報

画像

  • Western blot - Anti-ARFRP1/ARP antibody [EPR3899] (ab108199)
    Western blot - Anti-ARFRP1/ARP antibody [EPR3899] (ab108199)
    All lanes : Anti-ARFRP1/ARP antibody [EPR3899] (ab108199) at 1/1000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 3 : Fetal liver lysate
    Lane 4 : Ovary cancer lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 23 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ARFRP1/ARP antibody [EPR3899] (ab108199)
    Immunocytochemistry/ Immunofluorescence - Anti-ARFRP1/ARP antibody [EPR3899] (ab108199)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling ARFRP1/ARP with purified ab108199 at 1/500 dilution (4.8 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

  • Anti-ARFRP1/ARP antibody [EPR3899] (ab108199)
    Anti-ARFRP1/ARP antibody [EPR3899] (ab108199)

参考文献 (3)

ab108199 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108199 は 3 報の論文で使用されています。

  • Wilhelmi I  et al. The ARFRP1-dependent Golgi scaffolding protein GOPC is required for insulin secretion from pancreatic ß-cells. Mol Metab 45:101151 (2021). PubMed: 33359402
  • Rödiger M  et al. Adiponectin release and insulin receptor targeting share trans-Golgi-dependent endosomal trafficking routes. Mol Metab 8:167-179 (2018). WB, ICC/IF ; Human . PubMed: 29203237
  • Covington JD  et al. Skeletal muscle perilipin 3 and coatomer proteins are increased following exercise and are associated with fat oxidation. PLoS One 9:e91675 (2014). WB ; Human . PubMed: 24632837

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