AB108199

Anti-ARFRP1/ARP 抗体 [EPR3899]

Anti-ARFRP1/ARP antibody [EPR3899]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal ARFRP1/ARP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

別名を表示する

ARP1, ARFRP1, ADP-ribosylation factor-related protein 1, ARF-related protein 1, ARP

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ARFRP1/ARP antibody [EPR3899] (AB108199)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ARFRP1/ARP antibody [EPR3899] (AB108199)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling ARFRP1/ARP with purified ab108199 at 1/500 dilution (4.8 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL) was used as the secondary antibody only control.

Unknown

Western blot - Anti-ARFRP1/ARP antibody [EPR3899] (AB108199)

All lanes:

Western blot - Anti-ARFRP1/ARP antibody [EPR3899] (ab108199) at 1/1000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 10 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg

Lane 3:

Fetal liver lysate at 10 µg

Lane 4:

Ovary cancer lysate at 10 µg

Predicted band size: 23 kDa

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR3899

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The target ARFRP1 also known as ARP functions mechanistically as a small GTPase belonging to the ARF family. This protein possesses an approximate molecular mass of 20 kDa. It is ubiquitously expressed in various tissues exhibiting a significant presence in the cytoplasm and membrane compartments. ARFRP1 plays a role in regulating membrane trafficking and lipid metabolism influencing the formation and function of vesicles within the cell.

Biological function summary

ARFRP1 participates in intracellular vesicle transport and is critical for maintaining cellular homeostasis. It forms part of a complex that interacts with other proteins involved in vesicular traffic affecting cargo selection and vesicle formation. ARFRP1 contributes to maintaining the structural integrity of the Golgi apparatus and endosomes influencing the distribution of proteins and lipids across cellular compartments.

Pathways

ARFRP1 features prominently in pathways related to endocytic and exocytic transport. In the context of membrane traffic it closely interacts with proteins such as ARL1 and ARL4 which also play roles in these pathways. ARFRP1 influences the functioning of these pathways contributing to both protein recycling and secretion processes.

The dysfunction of ARFRP1 associates with Charcot-Marie-Tooth disease and Bardet-Biedl syndrome. In these conditions ARFRP1 may interact with related proteins like ARF6 impacting cell signaling and membrane dynamics. These interactions can lead to defects in cell transport mechanisms further elucidating the role of ARFRP1 in pathological states.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Trans-Golgi-associated GTPase that regulates protein sorting. Controls the targeting of ARL1 and its effector to the trans-Golgi. Required for the lipidation of chylomicrons in the intestine and required for VLDL lipidation in the liver.

See full target information ARFRP1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 45:101151 PubMed33359402

2020

The ARFRP1-dependent Golgi scaffolding protein GOPC is required for insulin secretion from pancreatic β-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ilka Wilhelmi,Stephan Grunwald,Niclas Gimber,Oliver Popp,Gunnar Dittmar,Anup Arumughan,Erich E Wanker,Thomas Laeger,Jan Schmoranzer,Oliver Daumke,Annette Schürmann

Molecular metabolism 8:167-179 PubMed29203237

2017

Adiponectin release and insulin receptor targeting share trans-Golgi-dependent endosomal trafficking routes.

Applications

ICC/IF, WB

Species

Human, Human

Maria Rödiger,Martin W Werno,Ilka Wilhelmi,Christian Baumeier,Deike Hesse,Nina Wettschureck,Stefan Offermanns,Kyungyeun Song,Michael Krauß,Annette Schürmann

PloS one 9:e91675 PubMed24632837

2014

Skeletal muscle perilipin 3 and coatomer proteins are increased following exercise and are associated with fat oxidation.

Applications

Species

Human

Jeffrey D Covington,Jose E Galgani,Cedric Moro,Jamie M LaGrange,Zhengyu Zhang,Arild C Rustan,Eric Ravussin,Sudip Bajpeyi

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARFRP1/ARP 抗体 [EPR3899]

Anti-ARFRP1/ARP antibody [EPR3899]

Immunocytochemistry/ Immunofluorescence - Anti-ARFRP1/ARP antibody [EPR3899] (AB108199)

Western blot - Anti-ARFRP1/ARP antibody [EPR3899] (AB108199)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise