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リコンビナントRabMAb

Anti-ARFGEF2/BIG2 抗体 [EPR10049] (ab180186)

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Western blot - Anti-ARFGEF2/BIG2 antibody [EPR10049] (ab180186)
  • Anti-ARFGEF2/BIG2 antibody [EPR10049] (ab180186)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10049] to ARFGEF2/BIG2
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ARFGEF2/BIG2 antibody [EPR10049]
    ARFGEF2/BIG2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10049] to ARFGEF2/BIG2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human ARFGEF2/BIG2 aa 1500-1600 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y6D5

  • ポジティブ･コントロール

    • HeLa, HepG2 and 293T cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab180186の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 202 kDa.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 202 kDa.
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

ターゲット情報

  • 関連性

    ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6 and the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.

  • 細胞内局在

    Cytoplasm. Membrane. Golgi apparatus. Cytoplasm › perinuclear region. Golgi apparatus › trans-Golgi network By similarity. Endosome By similarity. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cell projection › dendrite By similarity. Cytoplasmic vesicle By similarity. Cell junction › synapse By similarity. Cytoplasm › cytoskeleton By similarity. Note: Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.

  • 参照データベース

    • 別名

      • ADP ribosylation factor guanine nucleotide exchange factor 2 (brefeldin A inhibited) antibody
      • ADP ribosylation factor guanine nucleotide exchange factor 2 antibody
      • ARFGEF 2 antibody
      • ARFGEF2 antibody
      • ARFGEP2 antibody
      • BIG 2 antibody
      • BIG2 antibody
      • Brefeldin A inhibited 2 antibody
      • Brefeldin A inhibited GEP 2 antibody
      • Brefeldin A inhibited guanine nucleotide exchange protein 2 antibody
      • dJ1164I10.1 antibody
      • FLJ23723 antibody
      see all

    画像

    • Western blot - Anti-ARFGEF2/BIG2 antibody [EPR10049] (ab180186)
      Western blot - Anti-ARFGEF2/BIG2 antibody [EPR10049] (ab180186)
      All lanes : Anti-ARFGEF2/BIG2 antibody [EPR10049] (ab180186) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 202 kDa

    • Anti-ARFGEF2/BIG2 antibody [EPR10049] (ab180186)
      Anti-ARFGEF2/BIG2 antibody [EPR10049] (ab180186)

    参考文献 (0)

    ab180186 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab180186 は論文での使用が確認できていません。

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