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リコンビナントRabMAb

Anti-ARFGAP1 抗体 [EPR13650] (ab183746)

Submit a review Submit a question References (1)
Western blot - Anti-ARFGAP1 antibody [EPR13650] (ab183746)
  • Western blot - Anti-ARFGAP1 antibody [EPR13650] (ab183746)
  • Immunocytochemistry/ Immunofluorescence - Anti-ARFGAP1 antibody [EPR13650] (ab183746)
  • Immunocytochemistry/ Immunofluorescence - Anti-ARFGAP1 antibody [EPR13650] (ab183746)
  • Anti-ARFGAP1 antibody [EPR13650] (ab183746)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13650] to ARFGAP1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ARFGAP1 antibody [EPR13650]
    ARFGAP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13650] to ARFGAP1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human fetal brain lysate; HepG2, HeLa and MCF7 cell lysates; HeLa and MCF7 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab183746の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC/IF
1/500.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC/IF
1/500.

ターゲット情報

  • 機能

    GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and RNP24. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine.

  • 配列類似性

    Contains 1 Arf-GAP domain.

  • ドメイン

    The region downstream of Arf-GAP domain is essential to GAP activity in vivo. This region may be required for its targeting to Golgi membranes.

  • 細胞内局在

    Cytoplasm. Golgi apparatus. Associates with the Golgi complex.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ADP ribosylation factor 1 GTPase activating protein antibody
    • ADP ribosylation factor GTPase activating protein 1 antibody
    • ADP-ribosylation factor 1 GTPase-activating protein antibody
    • ADP-ribosylation factor GTPase-activating protein 1 antibody
    • ARF GAP 1 antibody
    • ARF1 directed GTPase activating protein antibody
    • ARF1 GAP antibody
    • ARF1-directed GTPase-activating protein antibody
    • ARF1GAP antibody
    • ARFG1_HUMAN antibody
    • Arfgap1 antibody
    • GAP protein antibody
    • HRIHFB2281 antibody
    see all

画像

  • Western blot - Anti-ARFGAP1 antibody [EPR13650] (ab183746)
    Western blot - Anti-ARFGAP1 antibody [EPR13650] (ab183746)
    All lanes : Anti-ARFGAP1 antibody [EPR13650] (ab183746) at 1/5000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • Western blot - Anti-ARFGAP1 antibody [EPR13650] (ab183746)
    Western blot - Anti-ARFGAP1 antibody [EPR13650] (ab183746)
    All lanes : Anti-ARFGAP1 antibody [EPR13650] (ab183746) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ARFGAP1 antibody [EPR13650] (ab183746)
    Immunocytochemistry/ Immunofluorescence - Anti-ARFGAP1 antibody [EPR13650] (ab183746)

    Immunofluorescent analysis of acetone-fixed HeLa cells labeling ARFGAP1 with ab183746 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-ARFGAP1 antibody [EPR13650] (ab183746)
    Immunocytochemistry/ Immunofluorescence - Anti-ARFGAP1 antibody [EPR13650] (ab183746)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling ARFGAP1 with ab183746 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).

  • Anti-ARFGAP1 antibody [EPR13650] (ab183746)
    Anti-ARFGAP1 antibody [EPR13650] (ab183746)

参考文献 (1)

ab183746 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab183746 は 1 報の論文で使用されています。

  • Chornyy S  et al. High abundance of ArfGAP1 found in the mossy fibers in hilus of the dentate gyrus region of the mouse brain. PLoS One 12:e0189659 (2017). PubMed: 29240824

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