AB248253

Anti-ARF1+ARF3 抗体 [EPR8304(B)] - BSA and Azide free

Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ARF3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication.

別名を表示する

ADP-ribosylation factor 3, ARF3, ADP-ribosylation factor 1, ARF1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (AB248253)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (AB248253)

This data was developed using ab129003, the same antibody clone in a different buffer formulation.

ab129003, at 1/50 dilution, staining ARF3 in paraffin-embedded Human stomach tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Western blot - Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (AB248253)

This data was developed using ab129003, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Human ARF-1 recombinant protein (ab123538), Human ARF-3 recombinant protein (ab113581).

All lanes:

Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (<a href='/products/primary-antibodies/arf1-arf3-antibody-epr8304b-ab129003'>ab129003</a>) at 1/20000 dilution

Lane 1:

Full length human ARF-1 recombinant protein at 0.015 µg

Lane 2:

Full length human ARF-3 recombinant protein at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 23 kDa

false

Exposure time: 3min

Unknown

Western blot - Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (AB248253)

This data was developed using ab129003, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (ab248253) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Human fetal brain lysate at 10 µg

Lane 4:

Raji cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR8304(B)

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

ab248253 is the carrier-free version of ab129003.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ARF1 and ARF3 are small GTP-binding proteins part of the ADP-ribosylation factor (ARF) family involved in vesicle formation and trafficking. Alternate names for these proteins include ADP-ribosylation factor 1 and 3. ARF1 has a molecular mass of about 21 kDa and ARF3 is similar in size. These proteins localize primarily to the Golgi apparatus and cytoplasmic regions including the endoplasmic reticulum and plasma membrane. The expression of ARF1 and ARF3 is widespread occurring in various cell types across different tissues indicating roles in fundamental cellular processes.

Biological function summary

ARF1 and ARF3 regulate the assembly of coat protein complexes on membrane surfaces which is essential for vesicle formation. They participate in the transport of proteins and lipids between cellular organelles particularly in the secretory and endocytic pathways. ARF1 specifically functions in activating phospholipase D and is part of large protein complexes that involve coatomer proteins and clathrin. While they share overlapping functions ARF1 and ARF3 exhibit some distinct roles in vesicle trafficking indicating the specificity in biological functions.

Pathways

ARF1 and ARF3 play important roles in the regulation of the Golgi apparatus and endocytic pathways. They are important in coordinating vesicular transport by interacting with proteins such as COPI and clathrin facilitating cargo sorting and trafficking. ARF1 is also linked to the phosphoinositide metabolic pathway influencing membrane dynamics and trafficking processes. Whereas ARF3 is involved in pathways that modulate cytoskeletal functions indicating its diverse effects within related cellular signaling and trafficking pathways.

Defects in ARF1 and ARF3 functions can contribute to conditions such as cancer and neurodegenerative diseases. Aberrant ARF1 expression has connections to cancer progression due to its influence on cell migration and invasion possibly linked with proteins like clathrin that modulate vesicular trafficking. Altered ARF3 activity associates with neurodegenerative disorders involving connections with proteins in signaling pathways related to ARF1 impacting neuronal transport and synaptic functions. These proteins' involvement in cellular trafficking highlights their roles in maintaining cellular homeostasis and their links to disease states.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

See full target information ADP-ribosylation factor 3

追加のターゲット

ARF1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 18:20220613 PubMed36694627

2023

circ-IARS depletion inhibits the progression of non-small-cell lung cancer by circ-IARS/miR-1252-5p/HDGF ceRNA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhua Yang,Chunping Yang,Ping Li

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARF1+ARF3 抗体 [EPR8304(B)] - BSA and Azide free

Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (AB248253)

Western blot - Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (AB248253)

Western blot - Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (AB248253)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (1)

Abcam product promise