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Anti-Aquaporin 4 抗体 [4/18] (ab9512)

Reviews (7)Q&A (11)References (48)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [4/18] (ab9512)

    Key features and details

    • Mouse monoclonal [4/18] to Aquaporin 4
    • Suitable for: IHC-P, IHC-Fr, IHC-FoFr, ELISA, WB
    • Reacts with: Mouse, Rat, Rabbit, Human, Zebrafish
    • Isotype: IgG3

    製品の概要

    • 製品名

      Anti-Aquaporin 4 antibody [4/18]
      Aquaporin 4 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [4/18] to Aquaporin 4

    • 由来種

      Mouse

    • アプリケーション

      適用あり: IHC-P, IHC-Fr, IHC-FoFr, ELISA, WBmore details

    • 種交差性

      交差種: Mouse, Rat, Rabbit, Human, Zebrafish
      交差が予測される動物種: Cow

    • 免疫原

      Synthetic peptide:

      VIDIDRGDEKKGKDSSGE

      , corresponding to amino acids 301-318 of Aquaporin 4
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • エピトープ

      Intracellular C-terminal AQP 4 epitope

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab9512の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P (4)
    1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
    IHC-Fr (1)
    Use at an assay dependent concentration.
    IHC-FoFr (2)
    Use at an assay dependent concentration. PubMed: 22438975
    ELISA
    Use at an assay dependent concentration. PubMed: 11595449
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 35 kDa. PubMed: 19831719
    特記事項
    IHC-P
    1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
    IHC-Fr
    Use at an assay dependent concentration.
    IHC-FoFr
    Use at an assay dependent concentration. PubMed: 22438975
    ELISA
    Use at an assay dependent concentration. PubMed: 11595449
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 35 kDa. PubMed: 19831719

    ターゲット情報

    • 機能

      Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.

    • 組織特異性

      Brain - muscle >> heart, kidney, lung, and trachea.

    • 配列類似性

      Belongs to the MIP/aquaporin (TC 1.A.8) family.

    • ドメイン

      Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

    • 翻訳後修飾

      Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40%.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • AQP 4 antibody
      • AQP-4 antibody
      • AQP4 antibody
      • AQP4_HUMAN antibody
      • Aquaporin type 4 antibody
      • Aquaporin-4 antibody
      • Aquaporin4 antibody
      • HMIWC 2 antibody
      • HMIWC2 antibody
      • Mercurial insensitive water channel antibody
      • Mercurial-insensitive water channel antibody
      • MGC22454 antibody
      • MIWC antibody
      • WCH 4 antibody
      • WCH4 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [4/18] (ab9512)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [4/18] (ab9512)
      Immunohistochemical staining of aquaporin 4 in rat brain.

    参考文献 (48)

    ab9512 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9512 は 48 報の論文で使用されています。

    • Wang C  et al. APOE-ε4 synergizes with sleep disruption to accelerate Aβ deposition and Aβ-associated tau seeding and spreading. J Clin Invest 133:N/A (2023). PubMed: 37279069
    • Wakid M  et al. Universal method for the isolation of microvessels from frozen brain tissue: A proof-of-concept multiomic investigation of the neurovasculature. Brain Behav Immun Health 34:100684 (2023). PubMed: 37822873
    • Karreman MA  et al. Active Remodeling of Capillary Endothelium via Cancer Cell-Derived MMP9 Promotes Metastatic Brain Colonization. Cancer Res 83:1299-1314 (2023). PubMed: 36652557
    • Sawicka E  et al. The Influence of Interaction between Cadmium with 17β-Estradiol, 2-Methoxyestradiol and 16α-Hydroxyestrone on Viability and p-Glycoprotein in Ovarian Cancer Cell Line. Int J Mol Sci 23:N/A (2022). PubMed: 35269769
    • Liu XL  et al. Mesenchymal Stem Cells Improve Cognitive Impairment and Reduce Aβ Deposition via Promoting AQP4 Polarity and Relieving Neuroinflammation in Rats With Chronic Hypertension-Induced Cerebral Small-Vessel Disease. Front Aging Neurosci 14:883503 (2022). PubMed: 35663575
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 18 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Aquaporin 4 antibody [4/18]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Cerebellum)
    Antigen retrieval step
    None
    Permeabilization
    Yes - PBS 1x, Triton X-100 1%
    Specification
    Cerebellum
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 May 24 2021

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Aquaporin 4 antibody [4/18]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - PBS 1x, Triton X-100 1%
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 May 24 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 4 antibody [4/18]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
    Permeabilization
    No
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Victor Bernal

    Verified customer

    投稿 Oct 27 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-Aquaporin 4 antibody [4/18]

     Good
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rabbit Tissue sections (Brain)
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    NDS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Govindaiah Vinukonda

    Verified customer

    投稿 Jul 09 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 4 antibody [4/18]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Bos taurus Tissue sections (kindey)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    Yes - saponin
    Specification
    kindey
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Katarzyna Michałek

    Verified customer

    投稿 Apr 18 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 4 antibody [4/18]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (intervertebral Disc)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    intervertebral Disc
    Blocking step
    1% BSA in 75% TBS 35% Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 25% · Temperature: 18°C
    Fixative
    10% Neutral Buffered Formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jul 14 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 4 antibody [4/18]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ph9 Microwave
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1500µg/mL · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    XXXXXX XXXXXX

    Verified customer

    投稿 Sep 06 2016

    Question

    Faint band first blot, primary antibody 1/500 overnight.
    I used Mouse as second antibody and I tested four times in different
    blots. In each one I tested actin and the bands were very strong. I guess
    that the problem is the AQP4.

    Read More
    Answer

    Thank you for this information. It is unusual for one to see a band and then subsequently see nothing, which is why I thought you may have been stripping and re-probing. You may improve your result by increasing the concentration of the primary antibody further. Generally the actin band will always be a lot stronger since it is a highly expressed loading control. We can send you a free of charge replacement of the antibody if you wish to try a new lot. Please let me know if this is satisfactory.

    Read More

    Question

    Inquiry: 1. Order details: - Batch number: GR16622-3 - Abcam product code: ab9512 - Antibody storage conditions: +4c 2. Please describe the problem (high background, wrong band size, more bands, no band etc). More bands 3. On what material are you testing the antibody in WB? - Species: rat - What’s cell line or tissue: brain cortex - Cell extract or Nuclear extract: cell extract - Purified protein or Recombinant protein: 3. The lysate - How much protein was loaded: 30ug - What lysis buffer was used: RIPA - What protease inhibitors were used: Roche protease inhibitors cocktail - What loading buffer was used: protein sample buffer - Phosphatase inhibitors: no - Did you heat the samples: 95ﾟC 10min 4. Electrophoresis/Gel conditions/ Transfer conditions - Reducing or non reducing gel: SDS-page - Reducing agent: 2-mercaptoethanol - Gel percentage : 12% - Transfer conditions: (Type of membrane, Protein transfer verified): semi-dry 5. Blocking conditions - Buffer: PBST - Blocking agent: milk, BSA, serum, what percentage: 5%milk - Incubation time: 1hr - Incubation temperature: room temp 6. Primary Antibody - Species: mouse - Reacts against: rat - At what dilution(s) have you tested this antibody:1:750 - What dilution buffer was used: PBST - Incubation time: overnight - Incubation temperature: 4ﾟC - What washing steps were done: 3 times per 5 min 7. Secondary Antibody - Species:goat - Reacts against: mouse - At what dilution(s) have you tested this antibody: 1:5000 - Incubation time: 1 hr - Wash steps: 5 times per 5 min - Fluorochrome or enzyme conjugate: HRP - Do you know whether the problems you are experiencing come from the secondary? no 8. Detection method ECl, ECl+, other detection method: ECL 9. Did you apply positive and negative controls along with the samples? Please specify. 10. Optimization attempts - How many times have you tried the Western? 5 - Have you eliminated the possibility that any background bands could be due to the secondary antibody? (Run a “No primary” control): yes - Do you obtain the same results every time e.g. are background bands always in the same place? yes - What steps have you altered? no

    Read More
    Answer

    Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

    Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab9512 Anti-Aquaporin 4 antibody [4/18]. I would also appreciate if you can confirm some further details:


    1. As this is a multipass membrane protein, which tent to aggregate in the loading buffer when boiled at high temperature due to their hydrophobic nature, I would like to recommend lowering the boiling temperature down to 60C, but keepingthe boiling time of10 min. In addition, use DTT instead of beta-mercaptoethanol as this kind of aggregation is favoured by b- ME.

    2. Please be aware that there are two isoforms of this protein, one is 29 and one is 35 kDa.

    3.For a better saturation of the membrane, I would recommend a blocking buffer containing 3% BSA for 2 hours at room temperature. BSA can sometimes give better results than milk.Please use the same agent during antibody incubation.

    3. In order to increase the specific binding, I would like to recommend decreasing the antibodies concentration down 1:1500.


    Should the suggestions not improve the results, please do let me know.

    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    I hope this information is helpful, and I thank you for your cooperation.

    Read More

    Question

    Thank you, that is useful, and we will probably go ahead with the peptide. I checked the price of the peptide, and it was quoted to me as $190, more than 3 times the cost of peptides we have ordered from other companies (santa cruz peptides cost ~$60). Is there a reason for this difference, are we able to order this peptide for a competitive price?   Here are 3 different competing peptide for competing antibodies and their price comparison.  FOXM1 (C-20) Psc-502 P (peptide) 100 µg/0.5 ml$61(73AUD) AR (N-20) Psc-816 P (peptide) 100 µg/0.5 ml$61(73AUD) PR (C-20) Psc-539 P (peptide) 100 µg/0.5 ml$61(73AUD)   Is there any way that we would be able to offer the customer a better price? ($190 is the AUD price for the customer).  

    Read More
    Answer

    Thank you for contacting us.

    We realize that our products can be considered to have premium price point. However, we feel that the product and service which we offer are of the highest possible quality. Under our Abpromise guarantee, ourproducts are guaranteed to work in the manner stated on the datasheetfor a period of six months. If you experience any difficulties using our product and if you have purchased this product within the last 180 days, we are happy to provide scientific support, refund or replacement.

    We do offer bulk discounts for orders of 10 units or more, and often run promotions for a number of product types. Please see our website or speak with customer service representative for more information about these.

    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

    Read More

    1-10 of 18 Abreviews or Q&A

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