Anti-Aquaporin 1 抗体 [EPR26554-46] (BSA and Azide free) (ab300464)
![Western blot - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)](https://www.abcam.co.jp/ps/products/300/ab300464/Images/AB300464-486557-anti-Aquaporin-1-EPR2655446-WB-mouse-rat.jpg "Western blot - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26554-46] to Aquaporin 1 - BSA and Azide free
- Suitable for: WB, IP, IHC-Fr, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free)
Aquaporin 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR26554-46] to Aquaporin 1 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Fr, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Mouse and rat kidney tissue lysate untreated and treated with Protein Deglycosylation MIX II; Human kidney and lung tissue lysate; Mouse kidney and lung tissue lysate; Rat kidney tissue lysate. IHC-p: Human kidney and pancreas tissue; Mouse kidney tissue; Rat kidney tissue. IHC-Fr: Mouse and rat kidney fresh frozen tissue. IP: Mouse kidney tissue lysate.
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特記事項
ab300464 is a carrier free version of ab300463.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR26554-46 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300464の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
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| IP |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa. |
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IP
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. -
組織特異性
Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver. -
配列類似性
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
ドメイン
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 358 Human
- Entrez Gene: 11826 Mouse
- Entrez Gene: 25240 Rat
- Omim: 107776 Human
- SwissProt: P29972 Human
- SwissProt: Q02013 Mouse
- SwissProt: P29975 Rat
- Unigene: 76152 Human
see all -
別名
- AQP 1 antibody
- AQP CHIP antibody
- AQP-1 antibody
see all
画像
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Western blot - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)All lanes : Anti-Aquaporin 1 antibody [EPR26554-46] (ab300463) at 1/1000 dilution
Lane 1 : Untreated Mouse kidney tissue lysate
Lane 2 : Mouse kidney tissue lysate treated with Protein Deglycosylation MIX II
Lane 3 : Untreated Rat kidney tissue lysate
Lane 4 : Rat kidney tissue lysate treated with Protein Deglycosylation MIX II
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28, 35-50 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondThis data was developed using ab300463, the same antibody clone in a different buffer formulation.
Blocking / Diluting buffer: 5% NFDM/TBST.
Aquaporin-1 is a glycosylated protein and can be deglycosylated by Protein Deglycosylation MIX II.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23238222).
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![Western blot - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)](https://www.abcam.co.jp/ps/products/300/ab300464/Images/AB300464-486556-anti-Aquaporin-1-EPR2655446-WB-human-mouse.jpg)
Western blot - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)All lanes : Anti-Aquaporin 1 antibody [EPR26554-46] (ab300463) at 1/1000 dilution
Lane 1 : Human kidney tissue lysate
Lane 2 : Human lung tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse lung tissue lysate
Lane 5 : Mouse brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/5000 dilution
Predicted band size: 28 kDa
Observed band size: 28,35-50 kDa why is the actual band size different from the predicted?This data was developed using ab300463, the same antibody clone in a different buffer formulation.
Blocking / Diluting buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23238222).
Low expression: mouse brain (PMID: 18487943).
Exposure time 7.75 seconds.
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![Western blot - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)](https://www.abcam.co.jp/ps/products/300/ab300464/Images/AB300464-486559-anti-Aquaporin-1-EPR2655446-wb-rat.jpg)
Western blot - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)All lanes : Anti-Aquaporin 1 antibody [EPR26554-46] (ab300463) at 1/1000 dilution
Lane 1 : Rat kidney tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/5000 dilution
Predicted band size: 28 kDa
Observed band size: 28,35-50 kDa why is the actual band size different from the predicted?This data was developed using ab300463, the same antibody clone in a different buffer formulation.
Blocking / Diluting buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23238222).
Low expression: rat brain (PMID: 18487943).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)](https://www.abcam.co.jp/ps/products/300/ab300464/Images/AB300464-486550-anti-Aquaporin-1-EPR2655446-ihc-human-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)This data was developed using ab300463, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Aquaporin 1 with ab300463 at 1/5000 dilution (0.091 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on human kidney. The section was incubated with ab300463 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)](https://www.abcam.co.jp/ps/products/300/ab300464/Images/AB300464-486551-anti-Aquaporin-1-EPR2655446-ihc-human-pancreas.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)This data was developed using ab300463, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Aquaporin 1 with ab300463 at 1/5000 dilution (0.091 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on human pancreas. The section was incubated with ab300463 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)](https://www.abcam.co.jp/ps/products/300/ab300464/Images/AB300464-486552-anti-Aquaporin-1-EPR2655446-ihc-mouse-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)This data was developed using ab300463, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Aquaporin 1 with ab300463 at 1/5000 dilution (0.091 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on mouse kidney. The section was incubated with ab300463 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)](https://www.abcam.co.jp/ps/products/300/ab300464/Images/AB300464-486553-anti-Aquaporin-1-EPR2655446-ihc-rat-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)This data was developed using ab300463, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Aquaporin 1 with ab300463 at 1/5000 dilution (0.091 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on rat kidney. The section was incubated with ab300463 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Frozen sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)](https://www.abcam.co.jp/ps/products/300/ab300464/Images/AB300464-486554-anti-Aquaporin-1-EPR2655446-ihcfr-mouse-kidney.jpg)
Immunohistochemistry (Frozen sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)This data was developed using ab300463, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized freshly frozen mouse kidney tissue labeling Aquaporin 1 with ab300463 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) at 1/1000 dilution (green). Positive staining on mouse kidney is observed. The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) at 1/1000 dilution.
No antigen retrieval was carried out.
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![Immunohistochemistry (Frozen sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)](https://www.abcam.co.jp/ps/products/300/ab300464/Images/AB300464-486555-anti-Aquaporin-1-EPR2655446-ihcfr-rat-kidney.jpg)
Immunohistochemistry (Frozen sections) - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)This data was developed using ab300463, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized freshly frozen rat kidney tissue labeling Aquaporin 1 with ab300463 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) at 1/1000 dilution (green). Positive staining on rat kidney is observed. The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) at 1/1000 dilution.
No antigen retrieval was carried out.
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![Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)](https://www.abcam.co.jp/ps/products/300/ab300464/Images/AB300464-486558-anti-Aquaporin-1-EPR2655446-wb-mouse.jpg)
Immunoprecipitation - Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)This data was developed using ab300463, the same antibody clone in a different buffer formulation.
Aquaporin 1 was immunoprecipitated from 10 μg mouse kidney tissue lysate with ab300463 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab300463 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: Mouse kidney tissue lysate 10 μg (Input).
Lane 2: Mouse kidney tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300521 in mouse kidney tissue lysate.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 5.5 seconds.
Observed MW(KDa): 28, 35-50.
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![Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)](https://www.abcam.co.jp/ps/products/300/ab300464/Images/AB300464-486539-Benefits-of-Recombinant-antibodies.jpg)
Anti-Aquaporin 1 antibody [EPR26554-46] (BSA and Azide free) (AB300464)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300464 は論文での使用が確認できていません。
