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リコンビナントRabMAb

Anti-APPBP1 抗体 [EPR15732] (ab187142)

Submit a review Submit a question References (3)
Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)
  • Flow Cytometry (Intracellular) - Anti-APPBP1 antibody [EPR15732] (ab187142)
  • Immunoprecipitation - Anti-APPBP1 antibody [EPR15732] (ab187142)
  • Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)
  • Anti-APPBP1 antibody [EPR15732] (ab187142)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15732] to APPBP1
  • Suitable for: Flow Cyt (Intra), WB, IP
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-APPBP1 antibody [EPR15732]
    APPBP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15732] to APPBP1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IPmore details

  • 種交差性

    交差種: Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa, A431, Human fetal muscle, Rat brain, Rat heart, Rat spleen and Human fetal brain lysates; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187142の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/120.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IP
1/30 - 1/40.
特記事項
Flow Cyt (Intra)
1/120.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IP
1/30 - 1/40.

ターゲット情報

  • 機能

    Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

  • 組織特異性

    Ubiquitous in fetal tissues. Expressed throughout the adult brain.

  • パスウェイ

    Protein modification; protein neddylation.

  • 配列類似性

    Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.

  • 細胞内局在

    Cell membrane. Colocalizes with APP in lipid rafts.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 59 kDa antibody
      • A 116A10.1 antibody
      • Amyloid beta precursor protein binding protein 1 59kD antibody
      • Amyloid beta precursor protein binding protein 1 59kDa antibody
      • Amyloid beta precursor protein binding protein 1 antibody
      • Amyloid beta precursor protein binding protein 1, 59 kDa antibody
      • Amyloid beta precursor protein-binding protein 1 antibody
      • Amyloid protein binding protein 1 antibody
      • Amyloid protein-binding protein 1 antibody
      • APP BP1 antibody
      • APP-BP1 antibody
      • APPBP 1 antibody
      • APPBP1 antibody
      • HPP 1 antibody
      • HPP1 antibody
      • NAE 1 antibody
      • NAE1 antibody
      • NEDD8 activating enzyme E1 regulatory subunit antibody
      • NEDD8 activating enzyme E1 subunit 1 antibody
      • NEDD8 activating enzyme E1 subunit antibody
      • NEDD8-activating enzyme E1 regulatory subunit antibody
      • Proto oncogene protein 1 antibody
      • Proto-oncogene protein 1 antibody
      • Protooncogene protein 1 antibody
      • ula 1 antibody
      • ULA1_HUMAN antibody
      see all

    画像

    • Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)
      Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)
      All lanes : Anti-APPBP1 antibody [EPR15732] (ab187142) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : Human fetal muscle tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 60 kDa

    • Flow Cytometry (Intracellular) - Anti-APPBP1 antibody [EPR15732] (ab187142)
      Flow Cytometry (Intracellular) - Anti-APPBP1 antibody [EPR15732] (ab187142)

      Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling APPBP1 with ab187142 at 1/120 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. 

       

       

    • Immunoprecipitation - Anti-APPBP1 antibody [EPR15732] (ab187142)
      Immunoprecipitation - Anti-APPBP1 antibody [EPR15732] (ab187142)

      Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab187142 at 1/50 dilution (lane 1) or PBS control (lane 2).
      Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    • Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)
      Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)
      All lanes : Anti-APPBP1 antibody [EPR15732] (ab187142) at 1/1000 dilution

      Lane 1 : Rat brain tissue lysate
      Lane 2 : Rat heart tissue lysate
      Lane 3 : Rat spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 60 kDa

    • Anti-APPBP1 antibody [EPR15732] (ab187142)
      Anti-APPBP1 antibody [EPR15732] (ab187142)

    参考文献 (3)

    ab187142 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab187142 は 3 報の論文で使用されています。

    • Xia X  et al. Neddylation of HER2 Inhibits its Protein Degradation and promotes Breast Cancer Progression. Int J Biol Sci 19:377-392 (2023). PubMed: 36632463
    • Gong TQ  et al. Proteome-centric cross-omics characterization and integrated network analyses of triple-negative breast cancer. Cell Rep 38:110460 (2022). PubMed: 35235781
    • Zeng Y  et al. An overactive neddylation pathway serves as a therapeutic target and MLN4924 enhances the anticancer activity of cisplatin in pancreatic cancer. Oncol Lett 18:2724-2732 (2019). PubMed: 31404297

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